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Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing

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Book cover Maize

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1676))

Abstract

DNA methylation plays an important role in the regulation of the expression of transposons and genes. Various methods have been developed to assay DNA methylation levels. Bisulfite sequencing is considered to be the “gold standard” for single-base resolution measurement of DNA methylation levels. Coupled with next-generation sequencing, whole-genome bisulfite sequencing (WGBS) allows DNA methylation to be evaluated at a genome-wide scale. Here, we described a protocol for WGBS in plant species with large genomes. This protocol has been successfully applied to assay genome-wide DNA methylation levels in maize and barley. This protocol has also been successfully coupled with sequence capture technology to assay DNA methylation levels in a targeted set of genomic regions.

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Acknowledgments

This work was supported by National Science Foundation Grants DBI-1237931 (to N.M.S.). Q.L. was supported by Huazhong Agricultural University Scientific and Technological Self-innovation Foundation (Program No. 2016RC012).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Qing Li

  2. Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, Saint Paul, MN, 55108, USA

    Qing Li, Peter J. Hermanson & Nathan M. Springer

Authors
  1. Qing Li
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  2. Peter J. Hermanson
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  3. Nathan M. Springer
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Corresponding author

Correspondence to Qing Li .

Editor information

Editors and Affiliations

  1. Department of Agronomy and Horticulture, University of Nebraska – Lincoln, Lincoln, Nebraska, USA

    L. Mark Lagrimini

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Li, Q., Hermanson, P.J., Springer, N.M. (2018). Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing. In: Lagrimini, L. (eds) Maize. Methods in Molecular Biology, vol 1676. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7315-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7315-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7314-9

  • Online ISBN: 978-1-4939-7315-6

  • eBook Packages: Springer Protocols

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