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Functional Genomics pp 337–349Cite as

Knockdown of Rice microRNA166 by Short Tandem Target Mimic (STTM)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1654))

Abstract

Small RNAs, including microRNAs (miRNAs), are abundant in plants and play key roles in controlling plant development and physiology. miRNAs regulate the expression of the target genes involved in key plant processes. Due to functional redundancy among miRNA family members in plants, an ideal approach to silence the expression of all members simultaneously, for their functional characterization, is desirable. Target mimic (TM) was the first approach to achieve this goal. Short tandem target mimic (STTM) is a potent approach complementing TM for silencing miRNAs in plants. STTMs have been successfully used in dicots to block miRNA functions. Here, we describe in detail the protocol for designing STTM construct to block miRNA functions in rice. Such approach can be applied to silence miRNAs in other monocots as well.

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Acknowledgements

This study was partially supported by funds from Henan Agricultural University (HAU) and NSFC (31571679), China. G.T. is supported by the National Science Foundation (NSF; grants IOS-1048216 and IOS-1340001). S.T. is supported by a postdoctoral scholarship from HAU. We are thankful to He Yi and Muhammad Uzair, Shanghai Jiao Tong University, China, for help in rice transformation and Joan Leonard, Ohio State University, Columbus, USA, for help in growing rice in greenhouse.

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Authors and Affiliations

  1. Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA

    Sachin Teotia & Guiliang Tang

  2. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Dabing Zhang

Authors
  1. Sachin Teotia
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  2. Dabing Zhang
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  3. Guiliang Tang
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Corresponding author

Correspondence to Guiliang Tang .

Editor information

Editors and Affiliations

  1. Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Michael Kaufmann

  2. Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Claudia Klinger

  3. Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Andreas Savelsbergh

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Teotia, S., Zhang, D., Tang, G. (2017). Knockdown of Rice microRNA166 by Short Tandem Target Mimic (STTM). In: Kaufmann, M., Klinger, C., Savelsbergh, A. (eds) Functional Genomics. Methods in Molecular Biology, vol 1654. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7231-9_25

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  • DOI: https://doi.org/10.1007/978-1-4939-7231-9_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7230-2

  • Online ISBN: 978-1-4939-7231-9

  • eBook Packages: Springer Protocols

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