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Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines

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Site-Specific Recombinases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1642))

Abstract

The retina is a thin neural tissue sitting on the backside of the eye, composed of light-sensing cells, interneurons, and output ganglion neurons. The latter send electrical signals to higher visual centers in the brain. Transgenic mouse lines are becoming one of the most valuable mammalian animal models for the study of visual signal processing within the retina. Especially, the generation of Cre recombinase transgenic mouse lines provides a powerful tool for genetic manipulation. A key step for the utilization of transgenic lines is the characterization of their transgene expression patterns in the retina. Here we describe a standard protocol for characterizing the expression pattern of the Cre recombinase or fluorescent proteins in the retina with an immunohistochemical approach.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 E. Canfield Avenue, Detroit, MI, 48201, USA

    Qi Lu Ph.D. & Zhuo-Hua Pan

  2. Department of Ophthalmology, Kresge Eye Institute, Wayne State University School of Medicine, Detroit, MI, USA

    Zhuo-Hua Pan

Authors
  1. Qi Lu Ph.D.
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  2. Zhuo-Hua Pan
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Correspondence to Qi Lu Ph.D. .

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Editors and Affiliations

  1. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA

    Nikolai Eroshenko

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Lu, Q., Pan, ZH. (2017). Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines. In: Eroshenko, N. (eds) Site-Specific Recombinases. Methods in Molecular Biology, vol 1642. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7169-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7169-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7167-1

  • Online ISBN: 978-1-4939-7169-5

  • eBook Packages: Springer Protocols

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