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Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology

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RNAi and Small Regulatory RNAs in Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1622))

Abstract

RNA interference (RNAi) technology is one of the main research tools in many studies of neural stem cells. This study describes effects of ATP11B on the morphology change of neural stem cells by using RNAi. ATP11B belongs to P4-ATPases family, which is preferential translocate phosphatidylserine of cell membrane. Although it exists in neural stem cells, its physiological function is poorly understood. By using RNAi technology to downregulate expression of ATP11B, we found distinct morphological changes in neural stem cells. More important, psiRNA-ATP11B-transfected cells displayed short neurite outgrowth compared to the control cells. These data strongly suggest that ATP11B plays a key role in the morphological change of neural stem cells.

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Acknowledgments

This project was sponsored by Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry and Natural Science Foundation of Shanghai (Grant No. 14ZR1414400), Young Eastern Scholar (Grant No. QD2015033), National Natural Science Foundation of China (Grant No. 31500827).

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Authors and Affiliations

  1. Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, 99 Shang Da Road, Shanghai, 200444, China

    Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang & Tieqiao Wen

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  1. Jiao Wang
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  2. Qian Wang
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  3. Fangfang Zhou
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  4. Dong Wang
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  5. Tieqiao Wen
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Corresponding author

Correspondence to Tieqiao Wen .

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Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, North Carolina, USA

    Baohong Zhang

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Wang, J., Wang, Q., Zhou, F., Wang, D., Wen, T. (2017). Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology. In: Zhang, B. (eds) RNAi and Small Regulatory RNAs in Stem Cells. Methods in Molecular Biology, vol 1622. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7108-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7108-4_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7106-0

  • Online ISBN: 978-1-4939-7108-4

  • eBook Packages: Springer Protocols

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