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Preparation of Platelet Concentrates for Research and Transfusion Purposes

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Serum/Plasma Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1619))

Abstract

Platelets are specialized cellular elements of the blood that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, 3086, Australia

    David W. Greening Ph.D. & Richard J. Simpson

  2. Transfusion Science, Melbourne, VIC, Australia

    Rosemary L. Sparrow Ph.D.

  3. Department of Immunology, Monash University, AMREP, 89 Commercial Road, Melbourne, VIC, 3004, Australia

    Rosemary L. Sparrow Ph.D.

Authors
  1. David W. Greening Ph.D.
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  2. Richard J. Simpson
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  3. Rosemary L. Sparrow Ph.D.
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Corresponding author

Correspondence to Rosemary L. Sparrow Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    David W. Greening

  2. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    Richard J. Simpson

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Greening, D.W., Simpson, R.J., Sparrow, R.L. (2017). Preparation of Platelet Concentrates for Research and Transfusion Purposes. In: Greening, D., Simpson, R. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 1619. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7057-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7057-5_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7056-8

  • Online ISBN: 978-1-4939-7057-5

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