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User Guide for the LORE1 Insertion Mutant Resource

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Plant Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1610))

Abstract

Lotus japonicus is a model legume used in the study of plant-microbe interactions, especially in the field of biological nitrogen fixation due to its ability to enter into a symbiotic relationship with a soil bacterium, Mesorhizobium loti. The LORE1 mutant population is a valuable resource for reverse genetics in L. japonicus due to its non-transgenic nature, high tagging efficiency, and low copy count. Here, we outline a workflow for identifying, ordering, and establishing homozygous LORE1 mutant lines for a gene of interest, LjFls2, including protocols for growth and genotyping of a segregating LORE1 population.

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Acknowledgments

This work is supported by the Danish National Research Foundation grant DNFR79.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, DK-8000, Aarhus C, Denmark

    Terry Mun, Anna Małolepszy, Niels Sandal, Jens Stougaard & Stig U. Andersen

Authors
  1. Terry Mun
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  2. Anna Małolepszy
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  3. Niels Sandal
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  4. Jens Stougaard
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  5. Stig U. Andersen
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Corresponding author

Correspondence to Stig U. Andersen .

Editor information

Editors and Affiliations

  1. Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria

    Wolfgang Busch

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Mun, T., Małolepszy, A., Sandal, N., Stougaard, J., Andersen, S.U. (2017). User Guide for the LORE1 Insertion Mutant Resource. In: Busch, W. (eds) Plant Genomics. Methods in Molecular Biology, vol 1610. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7003-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7003-2_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7001-8

  • Online ISBN: 978-1-4939-7003-2

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