Abstract
Telomeres terminate in 3′ single-stranded G-rich overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex specific nuclease (DSN) to digest all double-stranded DNA including telomeres to fragments of <10 bp, leaving the single-stranded overhangs intact. Their length can then be determined by Southern blot using super-sensitive telomere C-rich probes.
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Zhao, Y., Shay, J.W., Wright, W.E. (2017). Telomere G-Rich Overhang Length Measurement: DSN Method. In: Songyang, Z. (eds) Telomeres and Telomerase. Methods in Molecular Biology, vol 1587. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6892-3_5
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DOI: https://doi.org/10.1007/978-1-4939-6892-3_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6891-6
Online ISBN: 978-1-4939-6892-3
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