Abstract
T Cells can form very stable (synapses) or very transient and migratory (kinapses) contacts with antigen-presenting cells. Here, we describe how microchannels can be used to conveniently study the distinct dynamics of T cells during antigen recognition. Microchannels provide a controlled confined environment that promotes T cell migration and recapitulates kinapse and synapse behaviors when coated with appropriate pMHC molecules. We also depict the advantages of this in vitro approach for addressing mechanistic issues and for analysis.
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Acknowledgment
This work was supported by INSERM, Institut Curie, ANR-10-IDEX-0001-02 PSL*, ANR-11-LABX-0043 and ERC grant Strapacemi to AML-D, INSERM, Institut Pasteur and ERC starting grant LymphocyteContacts to P.B., and Association pour la Recherche sur le Cancer (ARC-PDF20140601095) to H.D.M.
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Moreau, H.D., Bousso, P., Lennon-Duménil, AM. (2017). Microchannels for the Study of T Cell Immunological Synapses and Kinapses. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_20
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DOI: https://doi.org/10.1007/978-1-4939-6881-7_20
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