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Analyzing Actin Dynamics at the Immunological Synapse

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The Immune Synapse

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1584))

Abstract

T cell signaling is inextricably linked to actin cytoskeletal dynamics at the immunological synapse (IS). This process can be imaged in living T cells expressing GFP actin or fluorescent F-actin binding proteins. Because of its planar nature, the IS provides a unique opportunity to image events as they happen, monitoring changes in actin retrograde flow in T cells interacting with different stimulatory surfaces or after pharmacological treatments. Here, we described the imaging methods and analytical procedures used to measure actin velocity across the IS in T cells spreading on planar stimulatory surfaces.

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Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia Research Institute, 3615 Civic Center Blvd, ARC 816D, Philadelphia, PA, 19104, USA

    Katarzyna I. Jankowska & Janis K. Burkhardt

  2. Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA, USA

    Katarzyna I. Jankowska & Janis K. Burkhardt

Authors
  1. Katarzyna I. Jankowska
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  2. Janis K. Burkhardt
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Corresponding author

Correspondence to Janis K. Burkhardt .

Editor information

Editors and Affiliations

  1. Department of Life sciences, University of Siena, Siena, Siena, Italy

    Cosima T. Baldari

  2. University of Oxford, Kennedy Institute of Rheumatology, Headington, Oxford, United Kingdom

    Michael L. Dustin

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Jankowska, K.I., Burkhardt, J.K. (2017). Analyzing Actin Dynamics at the Immunological Synapse. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6881-7_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6879-4

  • Online ISBN: 978-1-4939-6881-7

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