Abstract
Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.
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Acknowledgment
This work was supported by NIH grants R21AI094133 and R01AI083409 to Y.L., and R01AI093580 to H.L.
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Dhanwani, R., Ly, H., Liang, Y. (2017). Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform. In: Ferran, M., Skuse, G. (eds) Recombinant Virus Vaccines. Methods in Molecular Biology, vol 1581. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6869-5_10
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DOI: https://doi.org/10.1007/978-1-4939-6869-5_10
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