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Localized Surface Plasmon Resonance (LSPR)-Coupled Fiber-Optic Nanoprobe for the Detection of Protein Biomarkers

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Biosensors and Biodetection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1571))

Abstract

Here is presented a miniaturized, fiber-optic (FO) nanoprobe biosensor based on the localized surface plasmon resonance (LSPR) at the reusable dielectric-metallic hybrid interface with a robust, gold nano-disk array at the fiber end facet. The nanodisk array is directly fabricated using electron beam lithography (EBL) and metal lift-off process. The free prostate-specific antigen (f-PSA) has been detected with a mouse anti-human prostate-specific antigen (PSA) monoclonal antibody (mAb) as a specific receptor linked with a self-assembled monolayer (SAM) at the LSPR-FO facet surfaces. Experimental investigation and data analysis found near field refractive index (RI) sensitivity at ~226 nm/RIU with the LSPR-FO nanoprobe, and demonstrated the lowest limit of detection (LOD) at 100 fg/mL (~3 fM) of f-PSA in PBS solutions. The SAM shows insignificant nonspecific binding to the target biomarkers in the solution. The control experimentation using 5 mg/mL bovine serum albumin in PBS and nonspecific surface test shows the excellent specificity and selectivity in the detection of f-PSA in PBS. These results indicate important progress toward a miniaturized, multifunctional fiber-optic technology that integrates informational communication and sensing function for developing a high-performance, label-free, point-of-care (POC) device.

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Reference

  1. Mukundan H et al (2009) Optimizing a waveguide-based sandwich immunoassay for tumor biomarkers: evaluating fluorescent labels and functional surfaces. Bioconjug Chem 20(2):222–230

    Article  CAS  Google Scholar 

  2. Liu X et al (2008) A one-step homogeneous immunoassay for cancer biomarker detection using gold nanoparticle probes coupled with dynamic light scattering. J Am Chem Soc 130(9):2780–2782

    Article  CAS  Google Scholar 

  3. Sekhar PK, Ramgir NS, Bhansali S (2008) Metal-decorated silica nanowires: an active surface-enhanced raman substrate for cancer biomarker detection. J Phys Chem C 112(6):1729–1734

    Article  CAS  Google Scholar 

  4. Mani V et al (2009) Ultrasensitive immunosensor for cancer biomarker proteins using gold nanoparticle film electrodes and multienzyme-particle amplification. ACS Nano 3(3):585–594

    Article  CAS  Google Scholar 

  5. Henne WA et al (2006) Detection of folate binding protein with enhanced sensitivity using a functionalized quartz crystal microbalance sensor. Anal Chem 78(14):4880–4884

    Article  CAS  Google Scholar 

  6. Baker GA, Desikan R, Thundat T (2008) Label-free sugar detection using phenylboronic acid-functionalized piezoresistive microcantilevers. Anal Chem 80(13):4860–4865

    Article  CAS  Google Scholar 

  7. Lee HJ, Nedelkov D, Corn RM (2006) Surface plasmon resonance imaging measurements of antibody arrays for the multiplexed detection of low molecular weight protein biomarkers. Anal Chem 78(18):6504–6510

    Article  CAS  Google Scholar 

  8. Raether H (1988) Surface plasmons on smooth and rough surfaces and on gratings. Springer tracts in modern physics. Springer, Berlin

    Google Scholar 

  9. Leskova TA, Maradudin AA, Zierau W (2005) Surface plasmon polariton propagation near an index step. Optics Commun 249(1–3):23–35

    Article  CAS  Google Scholar 

  10. Jung LS et al (1998) Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films. Langmuir 14(19):5636–5648

    Article  CAS  Google Scholar 

  11. Mitsui K, Handa Y, Kajikawa K (2004) Optical fiber affinity biosensor based on localized surface plasmon resonance. Appl Phys Lett 85(18):4231–4233

    Article  CAS  Google Scholar 

  12. Chang T-C et al (2012) Using a fiber optic particle plasmon resonance biosensor to determine kinetic constants of antigen–antibody binding reaction. Anal Chem 85(1):245–250

    Article  Google Scholar 

  13. 1985. http://apps.who.int/iris/bitstream/10665/38405/1/WHO_TRS_725.pdf.

  14. Nishi Y, Doering R (2000) Handbook of semiconductor manufacturing technology. CRC Press, Boca Raton, FL

    Google Scholar 

  15. May GS, Spanos CJ (2006) Fundamentals of semiconductor manufacturing and process control. John Wiley & Sons, New York, NY

    Book  Google Scholar 

  16. Obando LA, Booksh KS (1999) Tuning dynamic range and sensitivity of white-light, multimode, fiber-optic surface plasmon resonance sensors. Anal Chem 71(22):5116–5122

    Article  CAS  Google Scholar 

  17. Wu H-J et al (2012) Membrane-protein binding measured with solution-phase plasmonic nanocube sensors. Nat Methods 9(12):1189–1191

    Article  CAS  Google Scholar 

  18. Willets KA, Van Duyne RP (2007) Localized surface plasmon resonance spectroscopy and sensing. Annu Rev Phys Chem 58(1):267–297

    Article  CAS  Google Scholar 

  19. Zhao J et al (2006) Localized surface plasmon resonance biosensors. Nanomedicine 1(2):219–228

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Nanoscience, Joint School of Nanoscience and Nanoengineering (JSNN), University of North Carolina at Greensboro, 2907 East Gate City Blvd, Greensboro, NC, 27410, USA

    Jianjun Wei & Zheng Zeng

  2. Center for Applied Optics, University of Alabama at Huntsville, Huntsville, AL, 35899, USA

    Yongbin Lin

Authors
  1. Jianjun Wei
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  2. Zheng Zeng
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  3. Yongbin Lin
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Corresponding authors

Correspondence to Jianjun Wei or Yongbin Lin .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

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Wei, J., Zeng, Z., Lin, Y. (2017). Localized Surface Plasmon Resonance (LSPR)-Coupled Fiber-Optic Nanoprobe for the Detection of Protein Biomarkers. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6848-0_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6846-6

  • Online ISBN: 978-1-4939-6848-0

  • eBook Packages: Springer Protocols

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