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Light Microscopy pp 151–165Cite as

Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1563))

Abstract

We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA

    Adam J. Trexler

  2. Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 3312, 50 South Drive, Bethesda, MD, 20892, USA

    Justin W. Taraska

Authors
  1. Adam J. Trexler
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  2. Justin W. Taraska
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Corresponding author

Correspondence to Justin W. Taraska .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California, USA

    Yolanda Markaki

  2. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    Hartmann Harz

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Trexler, A.J., Taraska, J.W. (2017). Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6810-7_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6808-4

  • Online ISBN: 978-1-4939-6810-7

  • eBook Packages: Springer Protocols

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