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Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders

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Multiplex Biomarker Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1546))

Abstract

Psychiatric disorders are complex diseases involving exogenous and endogenous factors. Biomarkers for diagnosis or prediction of successful treatment are not existent. In addition, the molecular basis of these diseases is still poorly understood. Blood plasma represents the most complex proteome as it contains subproteomes from several body tissues. However, the high abundance of some little proteins can obscure the analysis of hundreds of low abundance proteins, which are potential biomarkers. Therefore, removal of these high abundance proteins is pivotal in any proteomic study of plasma. Here, we present a method of depleting these proteins using immunoaffinity liquid chromatography.

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Acknowledgments

DMS was funded by FAPESP (São Paulo Research Foundation, grants 2013/08711-3, 2013/25702-8 and 2014/10068-4).

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Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato, 255, Campinas, SP, 13083-862, Brazil

    Sheila Garcia, Paulo A. Baldasso, Paul C. Guest & Daniel Martins-de-Souza

  2. UNICAMP’s Neurobiology Center, Campinas, Brazil

    Daniel Martins-de-Souza

Authors
  1. Sheila Garcia
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  2. Paulo A. Baldasso
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  3. Paul C. Guest
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  4. Daniel Martins-de-Souza
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Corresponding author

Correspondence to Daniel Martins-de-Souza .

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Editors and Affiliations

  1. Laboratory of Neuroproteomics, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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Garcia, S., Baldasso, P.A., Guest, P.C., Martins-de-Souza, D. (2017). Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 1546. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6730-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6730-8_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-6729-2

  • Online ISBN: 978-1-4939-6730-8

  • eBook Packages: Springer Protocols

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