Abstract
Chromatin immunoprecipitation (ChIP) is a sensitive, accurate, and reliable technique widely used to analyze protein-DNA interactions at specific binding sites in vivo. It has been a particularly powerful technique for mapping of histone modification patterns both at individual loci and genome-wide. Here we provide a detailed protocol for ChIP of histone modifications associated with active transcription in fission yeast (Schizosaccharomyces pombe).
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Mbogning, J., Tanny, J.C. (2017). Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast. In: Guillemette, B., Gaudreau, L. (eds) Histones. Methods in Molecular Biology, vol 1528. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6630-1_12
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DOI: https://doi.org/10.1007/978-1-4939-6630-1_12
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6628-8
Online ISBN: 978-1-4939-6630-1
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