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The Q-System: A Versatile Expression System for Drosophila

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Drosophila

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1478))

Abstract

Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression and genetic manipulations. In this review chapter, we focus on current state-of-the-art Q-system tools and reagents. We also discuss in vivo applications of the Q-system, together with GAL4/UAS and LexA/LexAop systems, for simultaneous expression of multiple effectors, intersectional labeling, and clonal analysis.

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Acknowledgements

We thank Chun-Chieh Lin, Qili Liu, Sha Liu, Darya Task and Olga Markova for their helpful comments on the manuscript.

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Authors and Affiliations

  1. The Solomon H. Snyder Department of Neuroscience, The Center for Sensory Biology, Johns Hopkins University School of Medicine, 855N. Wolfe St., Baltimore, MD, 21205, USA

    Olena Riabinina & Christopher J. Potter

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  1. Olena Riabinina
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  2. Christopher J. Potter
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Correspondence to Christopher J. Potter .

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Editors and Affiliations

  1. Institute of Genetics, Technische Universität Dresden, Dresden, Germany

    Christian Dahmann

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Riabinina, O., Potter, C.J. (2016). The Q-System: A Versatile Expression System for Drosophila . In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 1478. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6371-3_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6371-3_3

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