Abstract
Single-stranded oligonucleotides, or oligodeoxyribonucleotides (ODNs), are very important in several fields of science such as molecular biology, diagnostics, nanotechnology, and gene therapy. They are usually chemically synthesized. Here we describe an enzymatic method which enables us to synthesize pure oligonucleotides which can be up to several hundred long bases.
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Acknowledgement
We thank David and Astrid Hagelén Foundation and Svenska Sällskapet för Medicinsk Forskning (SSMF) for funding of CD; Vetenskapsrådet (Grant 2013-5883) and Stiftelsen för Strategisk Forskning (SSF, grant FFL12-0219) to B.H. Funding for open access charge: Vetenskapsrådet (Swedish Research Council).
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Ducani, C., Högberg, B. (2017). Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides. In: Hughes, R. (eds) Synthetic DNA. Methods in Molecular Biology, vol 1472. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6343-0_7
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DOI: https://doi.org/10.1007/978-1-4939-6343-0_7
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