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Meiosis pp 289–303Cite as

Analysis of Chromatin Dynamics During Drosophila Spermatogenesis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1471))

Abstract

In the course of spermatogenesis, germ cells undergo dramatic morphological changes that affect almost all cellular components. Therefore, it is impossible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating Drosophila male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat Drosophila sperm before immunofluorescence staining to help reveal epitopes in the highly condensed sperm chromatin that otherwise may be inaccessible to antibodies.

*Authors contributed equally.

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Acknowledgment

This work was supported by the German Research Foundation (DFG) within the TRR 81 and a research grant to CR (RA 2150/2-1). DM and ZE-G were supported by REPRO-TRAIN Marie Curie Initial Training Network (PITN-2011-289880).

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    Authors and Affiliations

    1. Department of Biology, Philipps-University Marburg, Karl-von-Frisch-Straße 8, 35043, Marburg, Germany

      Tim Hundertmark, Ina Theofel, Zeynep Eren-Ghiani & Christina Rathke

    2. Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, LS2 9JT, West Yorkshire, UK

      David Miller

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    1. Tim Hundertmark
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    2. Ina Theofel
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    3. Zeynep Eren-Ghiani
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    4. David Miller
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    5. Christina Rathke
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    Corresponding author

    Correspondence to Christina Rathke .

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    Editors and Affiliations

    1. Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada

      David T. Stuart

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    Hundertmark, T., Theofel, I., Eren-Ghiani, Z., Miller, D., Rathke, C. (2017). Analysis of Chromatin Dynamics During Drosophila Spermatogenesis. In: Stuart, D. (eds) Meiosis. Methods in Molecular Biology, vol 1471. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6340-9_17

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    • DOI: https://doi.org/10.1007/978-1-4939-6340-9_17

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-6338-6

    • Online ISBN: 978-1-4939-6340-9

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