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Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas

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Cilia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1454))

Abstract

Chlamydomonas reinhardtii is an outstanding model genetic organism for study of assembly of cilia. Here, methods are described for synchronization of ciliary regeneration in Chlamydomonas to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these methods can contribute to discovery of conserved genes that when defective lead to abnormal ciliary assembly and human disease.

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Acknowledgements

This work was supported by grants from the NIH (GM051173, WSS; Training Grant K12 GM000680, LMA; Training Grant 5T32 GM00836725, ELH) and the American Heart Association (14PRE19510013, ELH).

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Authors and Affiliations

  1. Department of Cell Biology, Emory University, 465 Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA, 30322, USA

    Emily L. Hunter, Winfield S. Sale & Lea M. Alford

Authors
  1. Emily L. Hunter
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  2. Winfield S. Sale
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  3. Lea M. Alford
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Corresponding author

Correspondence to Winfield S. Sale .

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Editors and Affiliations

  1. Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA

    Peter Satir

  2. Department of Biology, University of Copenhagen, Copenhagen, Denmark

    Søren Tvorup Christensen

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Hunter, E.L., Sale, W.S., Alford, L.M. (2016). Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas . In: Satir, P., Christensen, S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3789-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3789-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3787-5

  • Online ISBN: 978-1-4939-3789-9

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