Abstract
Metastasis is the major cause of breast cancer deaths. To spread from the primary tumor sites to distant tissues, solid tumor cells need to degrade the surrounding extracellular matrix (ECM). The protrusive membrane structures named invadopodia have been shown to play a critical role in the degradation of the ECM and invasion of invasive cancer cells. In this chapter, we describe a detailed protocol to examine invadopodia in human breast cancer cells.
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Acknowledgment
This work was supported by a research grant RP130425 from the Cancer Prevention and Research Institute of Texas (CPRIT) and a research grant R01HL119478 from the National Heart, Lung, and Blood Institute of the National Institutes of Health to GD. The content is solely the responsibility of the authors and does not necessarily represent the official views of the CPRIT and National Institutes of Health.
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Wang, Z., Liang, X., Cai, M., Du, G. (2016). Analysis of Invadopodia Formation in Breast Cancer Cells. In: Cao, J. (eds) Breast Cancer. Methods in Molecular Biology, vol 1406. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3444-7_18
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DOI: https://doi.org/10.1007/978-1-4939-3444-7_18
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