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Isolation and Characterization of Cancer Stem Cells from Primary Head and Neck Squamous Cell Carcinoma Tumors

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Cancer Drug Resistance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1395))

Abstract

Drug resistance remains a significant problem in the treatment of patients with head and neck squamous cell carcinoma (HNSCC). Recent reports showed that a subpopulation of highly tumorigenic cells, called cancer stem cells (CSCs), is uniquely resistant to chemotherapy, suggesting that these cells play an important role in the relapse of HNSCC. The development of methods for the isolation and culture of cancer stem cells is a key step to enable studies exploring the mechanisms underlying the role of these cells in chemoresistance. Here, we describe a method to isolate cancer stem cells from primary head and neck tumors and for the generation of orospheres.

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Author information

Authors and Affiliations

  1. Department of Restorative Sciences, University of Michigan School of Dentistry, Ann Arbor, MI, USA

    Hong S. Kim & Jacques E. Nör D.D.S., Ph.D.

  2. Division of Hematology/Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA

    Alexander T. Pearson M.D., Ph.D.

  3. Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI, USA

    Alexander T. Pearson M.D., Ph.D. & Jacques E. Nör D.D.S., Ph.D.

  4. Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, MI, USA

    Jacques E. Nör D.D.S., Ph.D.

  5. Department of Otolaryngology, University of Michigan School of Medicine, 1011 N. University Rm. 2309, Ann Arbor, MI, 48109-1078, USA

    Jacques E. Nör D.D.S., Ph.D.

Authors
  1. Hong S. Kim
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  2. Alexander T. Pearson M.D., Ph.D.
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  3. Jacques E. Nör D.D.S., Ph.D.
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Corresponding author

Correspondence to Jacques E. Nör D.D.S., Ph.D. .

Editor information

Editors and Affiliations

  1. New University of Lisbon, Lisbon, Portugal

    Jose Rueff

  2. New University of Lisbon, Lisbon, Portugal

    António Sebastião Rodrigues

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© 2016 Springer Science+Business Media New York

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Kim, H.S., Pearson, A.T., Nör, J.E. (2016). Isolation and Characterization of Cancer Stem Cells from Primary Head and Neck Squamous Cell Carcinoma Tumors. In: Rueff, J., Rodrigues, A. (eds) Cancer Drug Resistance. Methods in Molecular Biology, vol 1395. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3347-1_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3347-1_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3345-7

  • Online ISBN: 978-1-4939-3347-1

  • eBook Packages: Springer Protocols

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