Abstract
Stereotaxic surgery enables precise and consistent microinjections to discrete neural nuclei. Using stereotaxic surgery to deliver viral vectors is a powerful tool that provides the ability to manipulate gene expression in specific regions, or even specific cell types in the brain. Here, we describe the proper handling and stereotaxic delivery of recombinant adeno-associated virus to various neuroanatomical structures of the rodent brain.
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Acknowledgement
This work was supported by the Saint Mary’s Foundation.
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Benskey, M.J., Manfredsson, F.P. (2016). Intraparenchymal Stereotaxic Delivery of rAAV and Special Considerations in Vector Handling. In: Manfredsson, F. (eds) Gene Therapy for Neurological Disorders. Methods in Molecular Biology, vol 1382. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3271-9_14
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DOI: https://doi.org/10.1007/978-1-4939-3271-9_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3270-2
Online ISBN: 978-1-4939-3271-9
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