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Clinical Applications of Mass Spectrometry in Drug Analysis pp 167–175Cite as

Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1383))

Abstract

Ethyl glucuronide and ethyl sulfate are minor conjugated metabolites of ethanol that can be detected in urine for several days after last ingestion of ethanol. The monitoring of ethanol use has both clinical and forensic applications and a longer detection window afforded by monitoring these metabolites is obvious. LC-MS/MS is used to analyze diluted urine with deuterated analogs of each analyte as internal standards to ensure accurate quantitation and control for any potential matrix effects. High aqueous HPLC is used to chromatograph the metabolites. Negative ion electrospray is used to introduce the metabolites into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each metabolite, while a matrix-matched calibration curve is used for quantitation.

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Author information

Authors and Affiliations

  1. ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, 84108, UT, USA

    Matthew H. Slawson Ph.D.

  2. Department of Pathology, University of Utah Health Sciences Center, School of Medicine, ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, 84108, UT, USA

    Kamisha L. Johnson-Davis Ph.D.

  3. ARUP Laboratories, Salt Lake City, UT, USA

    Matthew H. Slawson Ph.D.

Authors
  1. Matthew H. Slawson Ph.D.
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  2. Kamisha L. Johnson-Davis Ph.D.
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Corresponding author

Correspondence to Kamisha L. Johnson-Davis Ph.D. .

Editor information

Editors and Affiliations

  1. Dept of Pathology & Lab Medicine, Children's Mercy Hospitals & Clinics, Kansas City, Missouri, USA

    Uttam Garg

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Slawson, M.H., Johnson-Davis, K.L. (2016). Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 1383. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3252-8_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3252-8_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3251-1

  • Online ISBN: 978-1-4939-3252-8

  • eBook Packages: Springer Protocols

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