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Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues

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Sample Preparation Techniques for Soil, Plant, and Animal Samples

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Isolation of the two most abundant plant macromolecules, lignin and cellulose, from the plant tissues is reviewed. Isolation methods depend on the plant tissue that is used as the source of the polymers. Assessment of purity has an important place in the whole isolation process. Extent of needed purity depends on the further application of the obtained polymers. Different lignin extraction procedures are presented. Lignin isolation via preceding extraction of pulp is usually applied on wood as a starting material. The procedure preceded by cell wall extraction and combined with lignin complexation with thioglicolic acid is used for plant tissues containing various kinds of cells or low lignin content comparing with protein content. Lignin purification with ionic liquids, as well as those obtaining Brauns’ native lignin, kraft lignin, or lignosulfonate are also described. The review of cellulose isolation procedures is presented, such as the alkaline procedure, as well as the method using ultrasound treatment, or the method applying enzyme technology. Dilute acid pretreatment in cellulose isolation is also depicted.

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Author information

Authors and Affiliations

  1. Life Sciences, Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, Belgrade, 11000, Serbia

    Ksenija Radotić D.Sc.

  2. Department of Engineering Design Technology, Cerritos College, Physical Sciences and Technology Building # PST225, 11110 Alondra Boulevard, Norwalk, CA, 92688, USA

    Miodrag Mićić D.Sc., Ph.D., M.T.M., C.S.W.P.

  3. Department of New Products Development, Cerritos College, Physical Sciences and Technology Building # PST225, 11110 Alondra Boulevard, Norwalk, CA, 92688, USA

    Miodrag Mićić D.Sc., Ph.D., M.T.M., C.S.W.P.

Authors
  1. Ksenija Radotić D.Sc.
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  2. Miodrag Mićić D.Sc., Ph.D., M.T.M., C.S.W.P.
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Correspondence to Ksenija Radotić D.Sc. .

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Editors and Affiliations

  1. Department of Engineering Design Technology, Cerritos College, Norwalk, California, USA

    Miodrag Micic

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Radotić, K., Mićić, M. (2016). Methods for Extraction and Purification of Lignin and Cellulose from Plant Tissues. In: Micic, M. (eds) Sample Preparation Techniques for Soil, Plant, and Animal Samples. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3185-9_26

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  • DOI: https://doi.org/10.1007/978-1-4939-3185-9_26

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3184-2

  • Online ISBN: 978-1-4939-3185-9

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