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Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation

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Book cover Estrogen Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1366))

Abstract

Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology.

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Acknowledgments

This work was supported by funding supplied by the NSF CAREER IOS-1053716 to N.V. and a Louisiana Board of Regents Fellowship to K.P.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Tulane University, 2000 Percival Stern Hall, New Orleans, LA, 70118, USA

    Sara Clark, Jennifer Rainville & Nandini Vasudevan

  2. The Neuroscience Program, Tulane University, New Orleans, LA, 70118, USA

    Kevin Pollard & Nandini Vasudevan

Authors
  1. Sara Clark
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  2. Kevin Pollard
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  3. Jennifer Rainville
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  4. Nandini Vasudevan
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Corresponding author

Correspondence to Nandini Vasudevan .

Editor information

Editors and Affiliations

  1. University of South Dakota Sanford School of Medicine, Vermillion, South Dakota, USA

    Kathleen M. Eyster

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Clark, S., Pollard, K., Rainville, J., Vasudevan, N. (2016). Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_36

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  • DOI: https://doi.org/10.1007/978-1-4939-3127-9_36

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3126-2

  • Online ISBN: 978-1-4939-3127-9

  • eBook Packages: Springer Protocols

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