Abstract
During the early studies of N-terminal arginylation, Edman degradation was widely used to identify N-terminally added Arg on protein substrates. This old method is reliable but highly depends on the purity and abundance of samples and can become misleading unless a highly purified, highly arginylated protein can be obtained. Here we report a mass spectrometry-based method that utilizes Edman degradation chemistry to identify arginylation in more complex and less abundant protein samples. This method can also apply to the analysis of other posttranslational modifications.
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References
Bradley CV, Williams DH, Hanley MR (1982) Peptide sequencing using the combination of edman degradation, carboxypeptidase digestion and fast atom bombardment mass spectrometry. Biochem Biophys Res Commun 104(4):1223–1230
Hunt DF, Yates JR III, Shabanowitz J, Winston S, Hauer CR (1986) Protein sequencing by tandem mass spectrometry. Proc Natl Acad Sci U S A 83(17):6233–6237
Wang J, Han X, Wong CC, Cheng H, Aslanian A, Xu T, Leavis P, Roder H, Hedstrom L, Yates JR III, Kashina A (2014) Arginyltransferase ATE1 catalyzes midchain arginylation of proteins at side chain carboxylates in vivo. Chem Biol 21(3):331–337
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Kashina, A.S., Yates, J.R. (2015). Analysis of Arginylated Peptides by Subtractive Edman Degradation. In: Kashina, A. (eds) Protein Arginylation. Methods in Molecular Biology, vol 1337. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2935-1_14
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DOI: https://doi.org/10.1007/978-1-4939-2935-1_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2934-4
Online ISBN: 978-1-4939-2935-1
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