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TALENs pp 27–42Cite as

The Development of TALE Nucleases for Biotechnology

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1338))

Abstract

The development of a facile genome engineering technology based on transcription activator-like effector nucleases (TALENs) has led to significant advances in diverse areas of science and medicine. In this review, we provide a broad overview of the development of TALENs and the use of this technology in basic science, biotechnology, and biomedical applications. This includes the discovery of DNA recognition by TALEs, engineering new TALE proteins to diverse targets, general advances in nuclease-based editing strategies, and challenges that are specific to various applications of the TALEN technology. We review examples of applying TALENs for studying gene function and regulation, generating disease models, and developing gene therapies. The current status of genome editing and future directions for other uses of these technologies are also discussed.

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Acknowledgements

This work was supported by a US National Institutes of Health (NIH) Director’s New Innovator Award (DP2OD008586), National Science Foundation (NSF) Faculty Early Career Development (CAREER) Award (CBET-1151035), NIH R01DA036865, NIH R21AR065956, NIH UH3TR000505, NIH P30AR066527, the Duke Coulter Translational Partnership, and an American Heart Association Scientist Development Grant (10SDG3060033). D.G.O. was supported by an American Heart Association Mid-Atlantic Affiliate Predoctoral Fellowship.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA

    David G. Ousterout

  2. Department of Biomedical Engineering, Duke University, Room 136 Hudson Hall, Box 90281, Durham, NC, 27708-0281, USA

    Charles A. Gersbach Ph.D.

  3. Center for Genomic and Computational Biology, Duke University, Durham, NC, 27708, USA

    Charles A. Gersbach Ph.D.

  4. Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC, 27710, USA

    Charles A. Gersbach Ph.D.

Authors
  1. David G. Ousterout
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  2. Charles A. Gersbach Ph.D.
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Correspondence to Charles A. Gersbach Ph.D. .

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  1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ralf Kühn

  2. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Wolfgang Wurst

  3. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Benedikt Wefers

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Ousterout, D.G., Gersbach, C.A. (2016). The Development of TALE Nucleases for Biotechnology. In: Kühn, R., Wurst, W., Wefers, B. (eds) TALENs. Methods in Molecular Biology, vol 1338. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2932-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2932-0_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2931-3

  • Online ISBN: 978-1-4939-2932-0

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