Abstract
Assessing the dynamics of individual membrane proteins in living cells is a powerful approach to investigate their assembly, mobility, and function. Here, we describe how to image single G protein-coupled receptors (GPCRs), both in the active and inactive state. This is achieved by combining labeling of GPCRs with bright organic fluorophores and fluorescent imaging by total internal reflection fluorescence microscopy. Using this method, individual tracks of single molecules can be analyzed in parallel with high spatial precision and with frame rates up to 50/s.
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Wagner, J., Sungkaworn, T., Heinze, K.G., Lohse, M.J., Calebiro, D. (2015). Single-Molecule Fluorescence Microscopy for the Analysis of Fast Receptor Dynamics. In: Filizola, M. (eds) G Protein-Coupled Receptors in Drug Discovery. Methods in Molecular Biology, vol 1335. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2914-6_5
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DOI: https://doi.org/10.1007/978-1-4939-2914-6_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2913-9
Online ISBN: 978-1-4939-2914-6
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