Abstract
PCR on genomic DNA isolated from lymphocyte populations is an invaluable technique to analyze T cell receptor (TCR) α and β gene rearrangements. Although this approach is powerful, it also has limitations that must be accounted for in experimental design and data interpretation. Here, we provide background required for understanding these limitations, and then outline standard PCR methods that can be used for analysis of TCRα and β gene rearrangements in mice.
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Acknowledgements
This work was supported by NIH grants T32 HG000046 (LJR), R37 GM41052 and R01 AI49934 (LC and MSK), R01 CA125195 and R01 CA136470 (CHB), and a Leukemia and Lymphoma Society Scholar Award (CHB).
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Rupp, L.J., Chen, L., Krangel, M.S., Bassing, C.H. (2016). Molecular Analysis of Mouse T Cell Receptor α and β Gene Rearrangements. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_16
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DOI: https://doi.org/10.1007/978-1-4939-2809-5_16
Publisher Name: Humana Press, New York, NY
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