Abstract
Gli2 and Gli3 respond to the Hedgehog (Hh) signal in mammals by undergoing posttranslational modifications and moving to the nucleus. The study of Gli proteins has been hampered by the fact that their overexpression in cells prevents their proper regulation. To address this issue, we have developed a method of rapid generation of stable cell lines expressing near-endogenous and approximately equal levels of wild-type and mutant Gli proteins. This method is applicable to the study of effects of various mutations on Gli protein modifications and activity.
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Acknowledgments
This work was supported by the OPUS grant from the Polish National Science Centre (grant 2014/13/B/NZ3/00909) to P.N., a grant from the US National Institutes of Health (R21 NS074091) to R.R., a Distinguished Scientist Award from the Sontag Foundation to R.R., a Scholar award from the Pew Foundation to R.R., and a Scholar award from the V Foundation for Cancer Research to R.R.
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Niewiadomski, P., Rohatgi, R. (2015). Rapid Screening of Gli2/3 Mutants Using the Flp-In System. In: Riobo, N. (eds) Hedgehog Signaling Protocols. Methods in Molecular Biology, vol 1322. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2772-2_11
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DOI: https://doi.org/10.1007/978-1-4939-2772-2_11
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2771-5
Online ISBN: 978-1-4939-2772-2
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