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Tilling by Sequencing

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Plant Functional Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1284))

Abstract

TILLING is a method to find mutations in a gene of interest by scanning amplicons from a mutagenized population for sequence changes, commonly a single nucleotide. In the past 5 years, mutation detection by sequencing has become increasingly popular. This chapter details the experimental flow for TILLING-by-Sequencing, highlighting the critical steps involved in tridimensional pooling of genomic DNA templates, preparation of libraries for high-throughput sequencing, and bioinformatic processing of the sequence data.

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Acknowledgments

This work was supported by the National Science Foundation (Plant Genome award no. DBI–0822383).

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Authors and Affiliations

  1. Department of Plant Biology and Genome Center, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA

    Helen Tsai, Kathie Ngo, Meric Lieberman, Victor Missirian & Luca Comai

Authors
  1. Helen Tsai
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  2. Kathie Ngo
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  3. Meric Lieberman
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  4. Victor Missirian
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  5. Luca Comai
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Corresponding author

Correspondence to Luca Comai .

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Editors and Affiliations

  1. Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA

    Jose M. Alonso

  2. Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA

    Anna N. Stepanova

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© 2015 Springer Science+Business Media New York

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Tsai, H., Ngo, K., Lieberman, M., Missirian, V., Comai, L. (2015). Tilling by Sequencing. In: Alonso, J., Stepanova, A. (eds) Plant Functional Genomics. Methods in Molecular Biology, vol 1284. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2444-8_18

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  • DOI: https://doi.org/10.1007/978-1-4939-2444-8_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2443-1

  • Online ISBN: 978-1-4939-2444-8

  • eBook Packages: Springer Protocols

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