Abstract
The diagnosis of gastrointestinal nematode infections in small ruminants is central to studying the biology and epidemiology of these parasites and underpins their control. Traditional methods of diagnosis are inaccurate, time-consuming and laborious. Here, we describe a step-by-step protocol for the molecular-based diagnosis of infections by real-time PCR.
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Acknowledgments
This research was supported through funds from the Australian Research Council (RBG and ARJ). Other support from Melbourne Water Corporation, the National Health and Medical Research Council, and the Alexander von Humboldt Foundation is gratefully acknowledged (RBG). FR was the recipient of scholarships from the University of Melbourne.
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Roeber, F., Jex, A.R., Gasser, R.B. (2015). A Real-Time PCR Assay for the Diagnosis of Gastrointestinal Nematode Infections of Small Ruminants. In: Cunha, M., Inácio, J. (eds) Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Methods in Molecular Biology, vol 1247. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2004-4_10
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DOI: https://doi.org/10.1007/978-1-4939-2004-4_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2003-7
Online ISBN: 978-1-4939-2004-4
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