Abstract
Cryopreservation is an essential technique to preserve stem cells, semipermanently sustaining their potentials. There are two main approaches of cryopreservation for human pluripotent stem cells (hPSCs). The first is the vitrification, which involves instantaneous freeze and thaw of hPSCs. The second is the conventional slow-cooling method and a rapid thaw. Both cryopreservation protocols have been standardized and optimized to yield high survivability of hPSCs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fujioka T, Yasuchika K, Nakamura Y et al (2004) A simple and efficient cryopreservation method for primate embryonic stem cells. Int J Dev Biol 48:1149–1154
Suemori H, Yasuchika K, Hasegawa K et al (2006) Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage. Biochem Biophys Res Commun 345:926–932
Li T, Mai Q, Gao J et al (2010) Cryopreservation of human embryonic stem cells with a new bulk vitrification method. Biol Reprod 82:848–853
Wagh V, Meganathan K, Jagtap S et al (2011) Effects of cryopreservation on the transcrip-tome of human embryonic stem cells after thawing and culturing. Stem Cell Rev 7:506–517
Miyazaki T, Nakatsuji N, Suemori H (2013) Optimization of slow cooling cryopreservation for human pluripotent stem cells. Genesis 52(1):49–55
Acknowledgements
This work was supported by New Energy and Industrial Technology Development Organization (NEDO), Japan; Ministry of Health, Labour and Welfare, Japan.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Miyazaki, T., Suemori, H. (2015). Cryopreservation of Human Pluripotent Stem Cells: A General Protocol. In: Rich, I. (eds) Stem Cell Protocols. Methods in Molecular Biology, vol 1235. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1785-3_9
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1785-3_9
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1784-6
Online ISBN: 978-1-4939-1785-3
eBook Packages: Springer Protocols