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Quantification of Catecholamine Uptake in Adult Cardiac Myocytes

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Book cover Nuclear G-Protein Coupled Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1234))

Abstract

In adult cardiac myocytes, multiple G protein-coupled receptors (GPCR) localize to and signal at the nucleus. These include endothelin B receptors, angiotensin type 1 and 2 receptors, β1- and β3-adrenergic receptors, and α1A- and α1B-adrenergic receptors. Initiation of signaling through nuclear GPCRs requires that ligands be produced within or transported into the cardiac myocytes, yet mechanisms whereby these ligands are produced or transported into cardiac myocytes are largely unclear. To activate nuclear adrenergic receptors in adult cardiac myocytes, uptake of endogenous catecholamines epinephrine and norepinephrine occurs via organic cation transporter 3 (OCT3), a member of the slc22a family of genes. This chapter details a method to detect and quantify catecholamine uptake in intact adult cardiac myocytes using a fluorescent-based catecholamine uptake assay.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, University of Minnesota, 6-120 Jackson Hall, 321 Church St. SE, Minneapolis, MN, 55455, USA

    Erika F. Dahl

  2. Novartis Animal Health US, Inc., 1447140th Street, Larchwood, IA, 51241, USA

    Casey D. Wright

  3. Department of Integrative Biology and Physiology, University of Minnesota, 2231 6th Street SE, Minneapolis, MN, 55455, USA

    Timothy D. O’Connell

Authors
  1. Erika F. Dahl
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  2. Casey D. Wright
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  3. Timothy D. O’Connell
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Corresponding author

Correspondence to Timothy D. O’Connell .

Editor information

Editors and Affiliations

  1. Montreal Heart Institute & Université de Montréal, Montreal, Québec, Canada

    Bruce G. Allen

  2. Pharmacology and Therapeutics, McGill University, Montreal, Québec, Canada

    Terence E. Hébert

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© 2015 Springer Science+Business Media New York

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Dahl, E.F., Wright, C.D., O’Connell, T.D. (2015). Quantification of Catecholamine Uptake in Adult Cardiac Myocytes. In: Allen, B., Hébert, T. (eds) Nuclear G-Protein Coupled Receptors. Methods in Molecular Biology, vol 1234. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1755-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-1755-6_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1754-9

  • Online ISBN: 978-1-4939-1755-6

  • eBook Packages: Springer Protocols

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