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Role of Glycosaminoglycans in Infectious Disease

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Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

Glycosaminoglycans (GAGs) have been shown to bind to a wide variety of microbial pathogens, including viruses, bacteria, parasites, and fungi in vitro. GAGs are thought to promote pathogenesis by facilitating pathogen attachment, invasion, or evasion of host defense mechanisms. However, the role of GAGs in infectious disease has not been extensively studied in vivo and therefore their pathophysiological significance and functions are largely unknown. Here we describe methods to directly investigate the role of GAGs in infections in vivo using mouse models of bacterial lung and corneal infection. The overall experimental strategy is to establish the importance and specificity of GAGs, define the essential structural features of GAGs, and identify a biological activity of GAGs that promotes pathogenesis.

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Acknowledgements

We would like to thank past and current members of the Park laboratory for developing essential reagents and constantly refining the described procedures. This work was supported by NIH grants R01 EY021765 and R01 HL107472.

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Authors and Affiliations

  1. Division of Respiratory Diseases, Children’s Hospital, Harvard Medical School, 320 Longwood Avenue, Enders-461, Boston, MA, 02115, USA

    Akiko Jinno & Pyong Woo Park Ph.D.

  2. Division of Newborn Medicine, Children’s Hospital, Harvard Medical School, Boston, MA, 02115, USA

    Pyong Woo Park Ph.D.

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  1. Akiko Jinno
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  2. Pyong Woo Park Ph.D.
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Correspondence to Pyong Woo Park Ph.D. .

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Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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Jinno, A., Park, P.W. (2015). Role of Glycosaminoglycans in Infectious Disease. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_45

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_45

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