Abstract
Bone is a regenerative tissue with an innate ability to self-remodel in response to environmental stimuli and the need to repair damage. Rodent models of fracture healing, and in particular genetic mouse models, can be used to study the contributions of specific molecular switches to skeletal repair, as well as to recreate and exacerbate biological development and repair mechanisms in postnatal skeletons. Here, we describe methodology for producing fully stabilized, single-cortex defects in mouse femurs to study mechanisms of intramembranous bone regeneration.
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Acknowledgements
The NIH (R01 DE020194, T32 AR056950, F32 AR60140) and the Mayo Clinic Center for Regenerative Medicine supported this work. The authors thank Keith Condon (Indiana University School of Medicine) for the Von Kossa/MacNeal’s tetrachrome staining protocol.
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McGee-Lawrence, M.E., Razidlo, D.F. (2015). Induction of Fully Stabilized Cortical Bone Defects to Study Intramembranous Bone Regeneration. In: Westendorf, J., van Wijnen, A. (eds) Osteoporosis and Osteoarthritis. Methods in Molecular Biology, vol 1226. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1619-1_14
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DOI: https://doi.org/10.1007/978-1-4939-1619-1_14
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