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Mitochondrial Metabolomics Using High-Resolution Fourier-Transform Mass Spectrometry

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Mass Spectrometry in Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1198))

Abstract

High-resolution Fourier-transform mass spectrometry (FTMS) provides important advantages in studies of metabolism because more than half of common intermediary metabolites can be measured in 10 min with minimal pre-detector separation and without ion dissociation. This capability allows unprecedented opportunity to study complex metabolic systems, such as mitochondria. Analysis of mouse liver mitochondria using FTMS with liquid chromatography shows that sex and genotypic differences in mitochondrial metabolism can be readily distinguished. Additionally, differences in mitochondrial function are readily measured, and many of the mitochondria-related metabolites are also measurable in plasma. Thus, application of high-resolution mass spectrometry provides an approach for integrated studies of complex metabolic processes of mitochondrial function and dysfunction in disease.

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Acknowledgements

This work was supported by NIH grants ES009047, ES023485 HL113451, AG038746, ES016731, and NIAID Contract HHSN2722 01200031C.

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Authors and Affiliations

  1. Clinical Biomarkers Laboratory, Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, 615 Michael Street, Atlanta, GA, 30322, USA

    Young-Mi Go, Karan Uppal, ViLinh Tran & James R. Roede

  2. Department of Civil and Environmental Engineering, Tufts University, Medford, MA, USA

    Douglas I. Walker & Kurt D. Pennell

  3. Bases Moléculaires et Structurales des Systèmes, Infectieux, UMR 5086, Université Lyon 1, Lyon, France

    Lauriane Dury & Hélène Baubichon-Cortay

  4. Chemistry Department, Emory University, Atlanta, GA, USA

    Frederick H. Strobel

  5. Division of Pulmonary, Allergy and Critical Care Medicine and Clinical Biomarkers Laboratory, Department of Medicine, Emory University, 615 Michael Street, Atlanta, GA, 30322, USA

    Dean P. Jones

Authors
  1. Young-Mi Go
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  2. Karan Uppal
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  3. Douglas I. Walker
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  4. ViLinh Tran
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  5. Lauriane Dury
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  6. Frederick H. Strobel
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  7. Hélène Baubichon-Cortay
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  8. Kurt D. Pennell
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  9. James R. Roede
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  10. Dean P. Jones
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Corresponding author

Correspondence to Dean P. Jones .

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Editors and Affiliations

  1. University of Washington Fred Hutchinson Cancer Rsrch Ctr, Seattle, Washington, USA

    Daniel Raftery

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Go, YM. et al. (2014). Mitochondrial Metabolomics Using High-Resolution Fourier-Transform Mass Spectrometry. In: Raftery, D. (eds) Mass Spectrometry in Metabolomics. Methods in Molecular Biology, vol 1198. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1258-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1258-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1257-5

  • Online ISBN: 978-1-4939-1258-2

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