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Procedures for Mucosal Immunization and Analyses of Cellular Immune Response to Candidate HIV Vaccines in Murine and Nonhuman Primate Models

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Immunoinformatics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1184))

Abstract

Sampling the mucosal tissues and analyses of immune responses are integral to vaccine-development strategies against human immunodeficiency virus (HIV), which is transmitted predominantly across the oro-genital mucosa. While immune assay development and standardization attempts employ mouse models, immunogenicity and protective efficacy that can be extrapolated to humans are realized only from experiments in nonhuman primates. Here, we describe commonly used practices for immunizations in rhesus macaques (Macaca mulatta) along with procedures for obtaining important mucosal tissues samples from macaques and mice. We also describe detailed protocols for two important assays applicable in mouse as well as primate experiments for determining antigen-specific T cells responses induced after vaccination.

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Author information

Authors and Affiliations

  1. Department of Immunology, The University of Texas MD Anderson Cancer Centre, Houston, TX, USA

    Shailbala Singh, Guojun Yang, Scott M. Anthony, Kimberly S. Schluns & K. Jagannadha Sastry

  2. Department of Veterinary Sciences, The University of Texas MD Anderson Cancer Centre, Bastrop, TX, USA

    Pramod Nehete, Patrick Hanley, Bharti Nehete & K. Jagannadha Sastry

  3. Stem Cell Transplantation Research, The University of Texas MD Anderson Cancer Centre, Houston, TX, USA

    Hong He

Authors
  1. Shailbala Singh
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  2. Pramod Nehete
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  3. Patrick Hanley
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  4. Bharti Nehete
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  5. Guojun Yang
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  6. Hong He
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  7. Scott M. Anthony
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  8. Kimberly S. Schluns
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  9. K. Jagannadha Sastry
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Corresponding author

Correspondence to K. Jagannadha Sastry .

Editor information

Editors and Affiliations

  1. Indian Statistical Institute, Kolkata, West Bengal, India

    Rajat K. De

  2. Indian Statistical Institute, Kolkata, West Bengal, India

    Namrata Tomar

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Singh, S. et al. (2014). Procedures for Mucosal Immunization and Analyses of Cellular Immune Response to Candidate HIV Vaccines in Murine and Nonhuman Primate Models. In: De, R., Tomar, N. (eds) Immunoinformatics. Methods in Molecular Biology, vol 1184. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1115-8_24

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  • DOI: https://doi.org/10.1007/978-1-4939-1115-8_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1114-1

  • Online ISBN: 978-1-4939-1115-8

  • eBook Packages: Springer Protocols

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