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Targeted Genome Modification via Triple Helix Formation

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Cancer Genomics and Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1176))

Abstract

Triplex-forming oligonucleotides (TFOs) are capable of coordinating genome modification in a targeted, site-specific manner, causing mutagenesis or even coordinating homologous recombination events. Here, we describe the use of TFOs such as peptide nucleic acids for targeted genome modification. We discuss this method and its applications and describe protocols for TFO design, delivery, and evaluation of activity in vitro and in vivo.

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Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, Yale University, New Haven, CT, USA

    Adele S. Ricciardi, Nicole A. McNeer, Kavitha K. Anandalingam & W. Mark Saltzman

  2. Department of Genetics, Yale University School of Medicine, 208040, New Haven, CT, USA

    Adele S. Ricciardi & Peter M. Glazer

  3. Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, USA

    Peter M. Glazer

Authors
  1. Adele S. Ricciardi
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  2. Nicole A. McNeer
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  3. Kavitha K. Anandalingam
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  4. W. Mark Saltzman
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  5. Peter M. Glazer
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Corresponding author

Correspondence to Peter M. Glazer .

Editor information

Editors and Affiliations

  1. Department of Pathology& Yale Cancer Center, Yale University School of Medicine, New Haven, Connecticut, USA

    Narendra Wajapeyee

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Ricciardi, A.S., McNeer, N.A., Anandalingam, K.K., Saltzman, W.M., Glazer, P.M. (2014). Targeted Genome Modification via Triple Helix Formation. In: Wajapeyee, N. (eds) Cancer Genomics and Proteomics. Methods in Molecular Biology, vol 1176. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0992-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-0992-6_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0991-9

  • Online ISBN: 978-1-4939-0992-6

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