Abstract
Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
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References
Beveridge WIB, Burnet FM (1946) The cultivation of viruses and rickettsiae in the chick embryo. His Majesty’s Stationery Office, London (Council MR, ed)
Merten OW, Hannoun C, Manuguerra JC, Ventre F, Petres S (1996) Production of influenza virus in cell cultures for vaccine preparation. Adv Exp Med Biol 397:141–151
Quinlivan M, Zamarin D, Garcia-Sastre A, Cullinane A, Chambers T, Palese P (2005) Attenuation of equine influenza viruses through truncations of the NS1 protein. J Virol 79:8431–8439
Yamagishi H, Ide S, Eiki T, Eiguchi Y, Nagamine T, Igarashi Y, Yoshioka I, Matumoto M (1984) Plaque assay of equine influenza virus. Vet Microbiol 9:187–192
Ilobi CP, Henfrey R, Robertson JS, Mumford JA, Erasmus BJ, Wood JM (1994) Antigenic and molecular characterization of host cell-mediated variants of equine H3N8 influenza viruses. J Gen Virol 75(Pt 3):669–673
Ilobi CP, Nicolson C, Taylor J, Mumford JA, Wood JM, Robertson JS (1998) Direct sequencing of the HA gene of clinical equine H3N8 influenza virus and comparison with laboratory derived viruses. Arch Virol 143:891–901
Cook RF, Cook SJ (1991) Differences in sensitivity in haemagglutinin inhibition assays between A/equine/H3N8 viruses isolated in eggs and MDCK cells are linked to cleavage of the haemagglutinin molecule. Vet Microbiol 27:253–261
Cook RF, Mumford JA, Douglas A, Wood JM (1988) The influence of the host cell on the antigenic properties of equine-2 influenza A viruses. In: Powell DG (ed) Equine infectious diseases v: proceedings of the fifth international conference. University Press of Kentucky, Lexington, pp 60–65
OIE (2012) 2.5.7. Equine influenza. In: Manual of diagnostic tests and vaccines for terrestrial animals, 7th edn. World Organisation for Animal Health (OIE), Paris, pp 865–878
Zhang L (2011) Differentiated innate immune responses correlate with the contrasting pathogenicity of the equine H7N7 influenza demonstrated in horses and Balb/C mice. University of Kentucky, Lexnington, KY
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Chambers, T.M., Reedy, S.E. (2014). Equine Influenza Culture Methods. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 1161. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0758-8_35
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DOI: https://doi.org/10.1007/978-1-4939-0758-8_35
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-0758-8
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