Abstract
Quantitative real-time PCR (qRT-PCR) is a common technique for mRNA quantification. Several methods have been developed in the past few years in order to adapt qRT-PCR also for small non-coding RNAs (sRNA). We here provide a simple and sensitive protocol that allows quantification of mRNAs, selected sRNAs, and long non-coding RNAs (lncRNA) in one cDNA sample by qRT-PCR.
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Acknowledgements
This work was supported by the DFG (LA2633-1/2) and the Max Planck Society (MPG)—Chemical Genomics Centre (CGC) through its supporting companies AstraZeneca, Bayer CropScience, Bayer Healthcare, Boehringer-Ingelheim, and Merck-Serono.
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Speth, C., Laubinger, S. (2014). Rapid and Parallel Quantification of Small and Large RNA Species. In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_6
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DOI: https://doi.org/10.1007/978-1-4939-0700-7_6
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