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The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines

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Herpes Simplex Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1144))

Abstract

DNA vaccines have been licensed in veterinary medicine and have promise for humans. This format is relatively immunogenic in mice and guinea pigs, the two principle HSV-2 animal models, permitting rapid assessment of vectors, antigens, adjuvants, and delivery systems. Limitations include the relatively poor immunogenicity of naked DNA in humans and the profound differences in HSV-2 pathogenesis between host species. Herein, we detail lessons learned over the last few years investigating candidate DNA vaccines in the progesterone-primed female mouse vaginal model of HSV-2 infection as a guide to investigators in the field.

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Author information

Authors and Affiliations

  1. Department of Medicine, University of Washington, 750 Republican Street, Room E651, Mail Stop 35806, Seattle, WA, 98195, USA

    Joshua O. Marshak, Lichun Dong & David M. Koelle M.D.

  2. Department of Laboratory Medicine, University of Washington, 750 Republican Street, Room E651, Mail Stop 35806, Seattle, WA, 98195, USA

    David M. Koelle M.D.

  3. Department of Global Health, University of Washington, 750 Republican Street, Room E651, Mail Stop 35806, Seattle, WA, 98195, USA

    David M. Koelle M.D.

  4. Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA

    David M. Koelle M.D.

  5. Benaroya Research Institute, Seattle, WA, 98109, USA

    David M. Koelle M.D.

Authors
  1. Joshua O. Marshak
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  2. Lichun Dong
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  3. David M. Koelle M.D.
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Corresponding author

Correspondence to David M. Koelle M.D. .

Editor information

Editors and Affiliations

  1. Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia

    Russell J. Diefenbach

  2. Institute of Virology, University of Zürich, Zürich, Switzerland

    Cornel Fraefel

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© 2014 Springer Science+Business Media New York

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Marshak, J.O., Dong, L., Koelle, D.M. (2014). The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus. Methods in Molecular Biology, vol 1144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0428-0_21

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  • DOI: https://doi.org/10.1007/978-1-4939-0428-0_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0427-3

  • Online ISBN: 978-1-4939-0428-0

  • eBook Packages: Springer Protocols

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