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Evaluating a Particular Circulating MicroRNA Species from an SLE Patient Using Stem-Loop qRT-PCR

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Systemic Lupus Erythematosus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1134))

Abstract

Systemic lupus erythematosus (SLE) is a complex autoimmune disease, and correct judgment of SLE activity is very important in guiding precise clinical treatment. Circulating microRNAs (miRNAs) could serve as potential biomarkers of disease activity or status in SLE, and here we describe a modified qRT-PCR method for detecting them. Stem loop has become one of the most powerful methods for determining miRNA expression because it is highly sensitive and accurate and requires only small amount of sample. In this chapter, we focus on a stem-loop reverse transcription-bound SYBR green qRT-PCR protocol for evaluating a particular circulating miRNA species in SLE patients.

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Author information

Authors and Affiliations

  1. Clinical Medical Research Centre, Second Clinical Medical School of Jinan University (Shenzhen People’s Hospital), Shenzhen, Guangdong, China

    Weiguo Sui, Fuhua Liu & Yong Dai

  2. Nephrology Department, Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, Guilin, Guangxi, China

    Jiejing Chen & Minglin Ou

Authors
  1. Weiguo Sui
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  2. Fuhua Liu
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  3. Jiejing Chen
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  4. Minglin Ou
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  5. Yong Dai
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Editor information

Editors and Affiliations

  1. University of Exeter Medical School, Devon, United Kingdom

    Paul Eggleton

  2. Section of Immunology and Infection, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

    Frank J. Ward

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© 2014 Springer Science+Business Media, New York

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Sui, W., Liu, F., Chen, J., Ou, M., Dai, Y. (2014). Evaluating a Particular Circulating MicroRNA Species from an SLE Patient Using Stem-Loop qRT-PCR. In: Eggleton, P., Ward, F. (eds) Systemic Lupus Erythematosus. Methods in Molecular Biology, vol 1134. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0326-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-0326-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0325-2

  • Online ISBN: 978-1-4939-0326-9

  • eBook Packages: Springer Protocols

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