Abstract
Tissue oxygen level is a critical determinant of both functionality and viability of cells and tissue in vivo. The oxygen level is highly regulated through complex, multilevel modulation of vascular resistance throughout the vascular tree. We have developed a method for oxygen measurement using oxygen dependent quenching of phosphorescence that is well suited for study of the regulation of tissue oxygenation in vivo. It is a minimally invasive optical method that makes it possible, in real time, to determine either mean oxygen pressure or entire histograms of the distribution of oxygen in the tissue microvasculature. When using near infrared phosphors, the measurements sample the blood volume throughout the tissue between the excitation and collection sites. By measuring phosphorescence lifetimes instead of intensity, interference by other pigments in the tissue that absorb or fluoresce at the measurement wavelengths is avoided. Since the strength of the signal is inversely related to the oxygen pressure, tissue regions with relatively low oxygen (hypoxia) can be readily identified. Calibration of the oxygen dependence of phosphorescence is absolute, eliminating the potential errors due to altered calibration, and the lifetime measurements do not «drift» over time of measurement.
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References
J.M. Vanderkooi, and D.F. Wilson, A new method for measuring oxygen concentration in biological systems. Adv. Exptl. Med. Biol. 200 pp. 189–193, 1986.
J.M. Vanderkooi, G. Maniara, G., T. J. Green, and Wilson, D.F., An optical method for measurement of dioxygen concentration based on quenching of phosphorescence. J. BioL Chem. 262, pp. 5476–5482, 1987.
D.F. Wilson, W.L. Rumsey, T.J. Green, and J.M. Vanderkooi, The oxygen dependence of mitochondria) oxidative phosphorylation measured by a new optical method for measuring oxygen. J. BioL Chem. 263, pp. 2712–2718, 1988.
T.J. Green, D.F. Wilson, J.M. Vanderkooi, and S.P. DeFeo, Phosphorimeters for analysis of decay profiles and real time monitoring of exponential decay and oxygen concentrations. Analytical Biochem. 174, pp. 73–79, 1989.
M. Pawlowski and D.F. Wilson, Monitoring of the oxygen pressure in the blood of live animals using the oxygen dependent quenching of phosphorescence. Adv. Exptl. Med. Biol. 316, pp. 179–185 1992.
J.R. Alcala, C. Yu, and G.J. Yeh, Digital phosphorimeter with frequency domain signal processing: Application to real-time fiber-optic oxygen sensing, Rev. Sci. Instrum., 64: pp. 1554–1560, 1993.
P.M. Gewehr and D.T. Delpy, Optical oxygen sensor based on phosphorescence lifetime quenching and employing a polymer immobilised metalloporphyrin probe. Part 1. Theory and instrumentation, Med. und Biol. Eng. und Comp., 31: pp. 2–10, 1993.
P.M. Gewehr, and D.T. Delpy, Optical oxygen sensor based on phosphorescence lifetime quenching and employing a polymer immobilised metalloporphyrin probe. Part 2. Sensor membranes and results, Med. und Biol. Eng. und Comp. 31, pp. 11–21, 1993.
S.A. Vinogradov, M.A. Femandez-Seara, B.W. Dugan, and D.F. Wilson, Frequency domain instrument for measuring phosphorescence lifetime distributions in heterogeneous samples. Review of Scientific Instruments, 72, pp. 3396–3406, 2001.
S.A. Vinogradov, and D.F. Wilson, Recursive maximum entropy algorithm and its application to the luminescence lifetime distribution recovery. J. Appl. Sped. 54, pp. 849–855, 2000.
W.L. Rumsey, J.M. Vanderkooi, and D.F. Wilson, Imaging of phosphorescence: A novel method for measuring the distribution of oxygen in perfused tissue. Science 241, pp. 1649–1651, 1988.
R.D. Shonat, D.F. Wilson, C.E. Riva, and M. Pawlowski, Oxygen distribution in the retinal and choroidal vessels of the cat as measured by a new phosphorescence imaging method. Applied Optics 31, pp. 3711–3718, 1992.
S.A. Vinogradov, L-W. Lo, and D.F. Wilson, Dendritic polyglutamic porphyrins: probing porphyrin protection by oxygen dependent quenching of phosphorescence. Chem. Eur. J., 5 (4), pp. 1338–1347, 1999.
L-W. Lo, S.A. Vinogradov, C.J. Koch, and D.F. Wilson, A new, water soluble, phosphor for oxygen measurements in vivo. Adv. Exptl. Med. Biol. 428, pp. 651–656, 1997.
S.A. Vinogradov and D.F. Wilson, “Dendrimers with Tetrabenzoporphyrin Core: Novel Near-IR Phosphors for 02 Measurements”, Abstr. Pap. ACS, 217, 50-INOR, U1122–U1122 Part 1 Mar 21 (1999).
V. Rozhkov, D.F. Wilson, and S.A. Vinogradov, Tuning oxygen quenching constants using dendritic encapsulation of phosphorescent Pd-porphyrins. Polymeric Materials: Science und Engineering 85, pp. 601–603, 2001.
V. Rozhkov, D.F. Wilson, and S.A. Vinogradov, Phosphorescent Pd porphyrin-dendrimers: tuning core accessibility by varying hydrophobicity of dendrimer matrix. Macromolecules, 35, pp. 1991–1993, 2002.
I. Dunphy, S.A. Vinogradov and D.F. Wilson, Oxyphor R2 and G2: new phosphors for measuring oxygen by oxygen dependent quenching of phosphorescence. Analy. Biochem. 2002, In press.
S.A. Vinogradov, L-W. Lo, W.T. Jenkins, S.M. Evans, C. Koch, and D.F. Wilson, Non invasive imaging of the distribution of oxygen in tissue in vivo using near infra-red phosphors. Biophys. J. 70, pp. 1609–1617, 1996.
L-W. Lo, C.J. Koch, and D.F. Wilson, Calibration of oxygen dependent quenching of the phosphorescence of Pd-meso-tetra-(4-carboxyphenyl) porphine: a phosphor with general application for measuring oxygen concentration in biological systems. Analy. Biochem. 236, pp. 153–160, 1996.
O. Finikova, A.V. Cheprakov, I.P. Beletskaya, and S.A. Vinogradov, An expedient synthesis of substituted tetraaryltetrabenzoporphyrins, Chem. Commun 1, pp. 261–262, 2001.
S.A. Vinogradov and D.F. Wilson, Porphyrin Compounds for Imaging Oxygen, U.S. Patent #6, 362, 175, 2002.
C.H. Barlow, D.A. Rorvik, and J.J. Kelly, Imaging epicardial oxygen, Ann. of Biomed. Eng., 26, pp. 76–85, 1998.
S.A. Vinogradov and D.F. Wilson, Phosphorescence lifetime analysis with a quadratic programming algorithm for determining quencher distributions in heterogeneous systems. Biophys. J. 67, pp. 2048–2059, 1994.
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Wilson, D.F., Vinogradov, S.A., Rozhkov, V., Creed, J., Rietveld, I., Pastuszko, A. (2003). Monitoring the Dynamics of Tissue Oxygenation in Vivo by Phosphorescence Quenching. In: Thorniley, M., Harrison, D.K., James, P.E. (eds) Oxygen Transport to Tissue XXV. Advances in Experimental Medicine and Biology, vol 540. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6125-2_1
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DOI: https://doi.org/10.1007/978-1-4757-6125-2_1
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