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The Molecular Basis for Cytolytic T Lymphocyte Function: Analysis with Blocking Monoclonal Antibodies

  • Chapter
Mechanisms of Cell-Mediated Cytotoxicity

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 146))

Abstract

Cytolytic T lymphocytes (CTL) were identified during the late 1960’s (1,2) and are thought to be important effector cells in immunity to viruses (3) and in allograft rejection (4), Despite considerable effort during the past decade, little has been learned about the biochemical basis for the killing event. Not only do the presumed molecular mediators remain unidentified, but fundamental physiological questions remain unanswered.

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References

  1. Brunner, K.T., J. Mauel, J.-C. Cerottini, H. Rudolf, and B. Chapuis. In vitro studies of cellular and humoral immunity induced by tumor allografts. In “Mechanisms of Inflammation Induced by Immune Reactions,” P.A. Miescher and P. Grabar, eds., pp. 342–357 (1967).

    Google Scholar 

  2. Berke G., G. Yagil, H. Ginsburg, and M. Feldman. Kinetic analysis of a graft reaction induced in cell culture. Immunol. 17:723–740 (1969).

    CAS  Google Scholar 

  3. Doherty, P.C., and R.M. Zinkernagel. T-cell-mediated immuno-pathology in viral infections. Transplant. Rev. 19:89–120 (1974).

    PubMed  CAS  Google Scholar 

  4. Cerottini, J.-C., and K.T. Brunner. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv. Irmnunol, 18:67–132 (1974).

    Article  CAS  Google Scholar 

  5. Martz, E. Early steps in specific tumor cell lysis by sensitized mouse T lymphocytes. II. Electrolyte permeability increase in the target cell membrane concomitant with programming for lysis. J. Immunol. 117:1023–1027 (1976).

    PubMed  CAS  Google Scholar 

  6. Sanderson, C.J. The mechanism of lymphocyte-mediated cytotoxicity. Biol Rev. 56:153–197 (1981).

    Article  PubMed  CAS  Google Scholar 

  7. Boyle, M.D.P., S.H. Ohaninan, and T. Borsos. Studies on the terminal stages of antibody-complement-mediated killing of a tumor cell. II. Inhibition of transformation of T to dead cells by 3’5’CAMP. J. Immunol. 116:1276–1279 (1976).

    PubMed  CAS  Google Scholar 

  8. Burakoff, S.J., E. Martz, and B. Benacerraf. Is the primary complement lesion insufficient for lysis? Failure of cells damaged under osmotic protection to lyse in EDTA or at low temperature after removal of osmotic protection. Clin. Immunol, and Immunopathol. 4:108–126 (1975).

    Article  CAS  Google Scholar 

  9. Martz, E. Mechanism of specific tumor cell lysis by allo-immune T-lymphocytes: Resolution and characterization of discrete steps in the cellular interaction. Contemp. Top. Immunobiol. 7:301–361 (1977).

    Article  PubMed  CAS  Google Scholar 

  10. Hiserodt, J.C., G.J. Tiangco, and G.A. Granger. The LT system in experimental animals. IV. Rapid specific lysis of Cr-labeled allogeneic target cells by highly unstable high MW lymphotoxin-receptor complex (es) released in vitro by activated alloimmune murine T lymphocytes. J. Immunol. 123:332–341 (1979).

    PubMed  CAS  Google Scholar 

  11. Martz, E., and B. Benacerraf. Multiple target cell killing by the cytolytic T-lymphocyte and the mechanism of cytotoxicity. Transplantation 21:5–11 (1976).

    Article  PubMed  CAS  Google Scholar 

  12. Zagury, D., J. Bernard, N. Thierness, M. Feldman, and G. Berke. Isolation and characterization of individual functionally reactive cytotoxic T lymphocytes: conjugation, killing and recycling at the single cell level. Eur. J. Immunol. 5:818–822 (1975).

    Article  Google Scholar 

  13. Golstein, P. Sensitivity of cytotoxic T cells to T-cell mediated cytotoxicity. Nature 252:81–83 (1974).

    Article  PubMed  CAS  Google Scholar 

  14. Küppers, R.C., and C.S. Henney. Evidence for direct linkage between antigen recogntion and lytic expression in effector T cells. J. Exp. Med. 143:684–689 (1976).

    Article  PubMed  Google Scholar 

  15. Bubbers, J.E., and C.S. Henney. Studies on the synthetic capacity and antigenic expression of glutaraldehyde-fixed target cells. J. Immunol. 114:1126–1131 (1975).

    PubMed  CAS  Google Scholar 

  16. Thorn R.M., and C.S. Henney. Studies on the mechanism of lymphocyte-mediated cytolysis. VI. A reappraisal of the requirement for protein synthesis during T cell-mediated lysis. J. Immunol. 116:146–149 (1976).

    PubMed  CAS  Google Scholar 

  17. Bevan, M.J., and M. Cohn. Cytotoxic effects of antigen-and mitogen-induced T cells on various targets. J. Immunol. 114:559–565 (1975).

    PubMed  CAS  Google Scholar 

  18. Gately, M., and E. Martz. Comparative studies on the mechanisms of nonspecific. Con A-dependent cytolysis and specific T cell-mediated cytolysis. J. Immunol. 119:1711–1722 (1977).

    PubMed  CAS  Google Scholar 

  19. Parker, W.L., and E. Martz. Lectin-induced nonlethal adhesions between cytolytic T lymphocytes and antigenically unrecognizable tumor cells, and nonspecific “triggering” of cytolysis. J. Immunol. 124:25–35 (1980).

    PubMed  CAS  Google Scholar 

  20. Küppers, R.C., and C.S. Henney. Studies on the mechanism of lymphocyte-mediated cytolysis. IX. Relationships between antigen recognition and lytic expression in killer T cells. J. Immunol. 118:71–76 (1977).

    PubMed  Google Scholar 

  21. Grimm, E., and B. Bonavida. Mechanism of cell-mediated cytotoxicity at the single cell level. I. Estimation of cytotoxic T lymphocyte frequency and relative lytic efficiency. J. Immunol. 123:2861–2869 (1979).

    PubMed  CAS  Google Scholar 

  22. Golstein, P., and E.T. Smith. The lethal hit stage of mouse T and non-T cell-mediated cytolysis. Differences in cation requirements and characterization of an analytical “cation pulse” method. Eur. J. Immunol. 6:31–37 (1976).

    Article  PubMed  CAS  Google Scholar 

  23. Wagner, H., and M. Rollinghoff. T cell-mediated cytotoxicity: Discrimination between antigen recognition, lethal hit and cytolysis phase. Eur. J. Immunol. 4:745–750 (1974).

    Article  PubMed  CAS  Google Scholar 

  24. Martz, E. Early steps in specific tumor cell lysis by sensitized mouse T-lymphocytes. I. Resolution and characterization. J. Immunol. 115:261–267 (1975).

    PubMed  CAS  Google Scholar 

  25. Martz, E. Immune lymphocyte to tumor cell adhesion: magnesium sufficient, calcium insufficient. J. Cell Biol. 84:584–598 (1980).

    Article  PubMed  CAS  Google Scholar 

  26. Gately, M.K., and E. Martz. Early steps in specific tumor cell lysis by sensitized mouse T lymphocytes. III. Resolution of two distinct roles for calcium in the cytolytic process. J. Immunol. 122:482–489 (1979).

    PubMed  CAS  Google Scholar 

  27. Plaut, M., J.E. Bubbers, and C.S. Henney. Studies on the mechanism of lymphocyte-mediated cytolysis. VII. Two stages in the T cell-mediated lytic cycle with distinct cation requirements. J. Immunol. 116:150–155 (1976).

    PubMed  CAS  Google Scholar 

  28. Golstein, P., C. Foa, and I.C.M. MacLennan. Mechanism of T-cell-mediated cytolysis: The differential impact of cyto-chalasins at the recognition and lethal hit stages. Eur. J. Immunol. 8:302–309 (1978).

    Article  PubMed  CAS  Google Scholar 

  29. Gately, M.K., and E. Martz. Inhibition of the lethal hit phase of T cell-mediated cytolysis by pharmacologic agents. Fed. Proc. 38:1166 (Abs. #4960) (1979).

    Google Scholar 

  30. Golstein, P. and E.T. Smith. Mechanism of T cell-mediated cytolysis: The lethal hit stage. Contemp. Top. Immunobiol. 7:273–300 (1977).

    Article  PubMed  CAS  Google Scholar 

  31. Martz E., C.D. Tsoukas, and W.J. Wechter. Evidence against Ca++ poisoning by killer cells: Mast cells killed by T lymphocytes do not secrete prelytically. J. Supramol. Struct. Suppl. 3:311 (Abstract 818) (1979).

    Google Scholar 

  32. Ko, L., and D. Lagunoff. Depletion of mast cell ATP inhibits complement-dependent cytotoxic histamine release. Exp. Cell Res. 100:313–321 (1976).

    Article  PubMed  CAS  Google Scholar 

  33. Henney, C.S. T cell mediated cytolysis: Consideration of the role of a soluble mediator. J. Reticuloendothelial Soc. 17:231–235 (1975).

    CAS  Google Scholar 

  34. Todd, R.F., and G. Berke. Functional characterization of membrane components of cytotoxic peritoneal exudate T lymphocytes. I. Search for T cell receptor activity in lymphocyte membrane fractions. Immunochemistry 11:313–320 (1974).

    Article  PubMed  CAS  Google Scholar 

  35. Whisnant, C.C., K.H. Singer, and D.B. Amos. Interaction of cytotoxic T lynnphocytes with target cells. I. Specific inhibition by detergent-solubilized, partially purified mouse histocompatibility anitgens. J. Immunol. 121:2253 (1978).

    PubMed  CAS  Google Scholar 

  36. Todd, R.F. III, R.D. Stuiting, and D.B. Amos. Lymphocyte-mediated cytolysis of allogeneic tumor cells in vitro. I. Search for target antigens in subcellular fractions. Cellular Immunol. 18:304–323 (1975).

    Article  CAS  Google Scholar 

  37. Nagy, Z.A., and B.E. Elliott. The receptor specificity of alloreactive T cells. Distinction between stimulator K, I, and D region products and degeneracy of third-party H-2 recognition by low-affinity T cells. J. Exp. Med. 150:1520–1537 (1979).

    Article  PubMed  CAS  Google Scholar 

  38. Linna, T.J., H.D. Engers, J.-C. Cerottini, and K.T. Brunner. Inhibition of cytolytic T lymphocyte activity with subcellular alloantigen preparations and with unlabeled allogenic target cells. J. Immunol. 120:1544–1549 (1978).

    PubMed  CAS  Google Scholar 

  39. Gilmer, P.J., H.O. McDevitt, and H.M. McConnell. Inhibition of specific T cell-target cell conjugates by target cell plasma membranes. J. Immunol. 120:774 (1978).

    PubMed  CAS  Google Scholar 

  40. Ozato, K., H.K. Ziegler, and C.S. Henney. Liposomes as model membrane systems for immune attack. I. Transfer of antigenic determinants to lymphocyte membranes after interactions with hapten-bearing liposomes. J. Immunol. 121:1376 (1978).

    PubMed  CAS  Google Scholar 

  41. Kimura, A.K., and H. Wigzell. Cell surface glycoproteins of murine cytotoxic T lymphocytes. I. T 145, a new surface glycoprotein selectively expressed on Ly l cytotoxic T lymphocytes. J. Exp. Med. 147:1418–1434 (1978).

    Article  PubMed  CAS  Google Scholar 

  42. Berke, G., Y. Kaufmann, and D. Gabison. Cell surface determinants of cytotoxic T lymphocytes: possible role in lymphocyte-target cell interaction. Fed. Proc. 39:1198 (Abstract #4891) (1980).

    Google Scholar 

  43. Gately, M.K., and E. Martz. Til: A new protein marker on activated murine T lymphocytes. J. Immunol. 126:709–714 (1981).

    PubMed  CAS  Google Scholar 

  44. Reif, A.E., and J.M. Allen. The AKR thymic antigen and its distribution in leukemias and nervous tissues. J. Exp. Med. 120:413 (1964).

    Article  PubMed  CAS  Google Scholar 

  45. Davignon, D., E. Martz, T. Reynolds, K. Kurzinger, and T.A. Springer. Lymphocyte function-associated antigen one (LFA-1): a surface antigen distinct from Lyt-2/3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78:4535–4539 (1981).

    Article  PubMed  CAS  Google Scholar 

  46. Kimura, A.K., and H. Wigzell. Cytotoxic T lymphocyte membrane components: An analysis of structures related to function. Cont. Top. Mol. Immunol. 6:209–244 (1977).

    CAS  Google Scholar 

  47. Redelman, D., and P.E. Trefts. In vitro studies of the rabbit immune system. VIII. Rabbit anti-mouse cytotoxic T-effector cells are inhibited by anti-rabbit T-cell serum in the absence of complement. J. Immunol. 121:1532–1539 (1978).

    PubMed  CAS  Google Scholar 

  48. Rabinowitz, R., and M. Schlesinger. Inhibition of the activity of cytotoxic murine T lymphocytes by antibodies to idiotypic determinants. Immunol. 39:93–99 (1980).

    CAS  Google Scholar 

  49. Kimura, A.K. Inhibition of specific cell-mediated cytotoxicity by anti-T-cell receptor antibody. J. Exp. Med. 139:888–901 (1974).

    Article  PubMed  CAS  Google Scholar 

  50. Rabinowitz, R., R. Laskov, and M. Schlesinger. Inhibition of cell-mediated lysis by xenoantibodies reactive with effector T lymphocytes. Eur. J. Immunol. 10:219–223 (1980).

    Article  PubMed  CAS  Google Scholar 

  51. Hiserodt, J.C., and B. Bonavida. Studies on the induction and expression of T cell-mediated immunity. XI. Inhibition of the “Lethal Hit” in T cell-mediated cytotoxicity by heterologous rat antiserum made against alloimmune cytotoxic T lymphocytes. J. Immunol. 126:256–262 (1981).

    PubMed  CAS  Google Scholar 

  52. Platsoucas, C.D., and R.A. Good. Inhibition of specific cell-mediated cytotoxicity by monoclonal antibodies to human T cell antigens. Proc. Natl. Acad. Sci. USA 78:4500–4504 (1981).

    Article  PubMed  CAS  Google Scholar 

  53. Chang, T.W., P.C. Kung, S.P. Gingras, and G. Goldstein. Does 0KT3 monoclonal antibody react with an antigen-recognition structure on human T cells? Proc. Natl. Acad. Sci. USA 78: 1805–1808 (1981).

    Article  PubMed  CAS  Google Scholar 

  54. Tsoukas, C.D., R.I. Fox, D.A. Carson, S. Fong, and J.H. Vaughan. Monoclonal antibody 0KT3 blocks the function of human cytotoxic T-lymphocytes against autologous EBV-transformed target cells. Submitted for publication.

    Google Scholar 

  55. Reinherz, E.I., R.E. Hussey, and S.F. Schlossman. A monoclonal antibody blocking htiman T cell function. Eur. J. Immunol. 10: 758–762 (1980).

    Article  PubMed  CAS  Google Scholar 

  56. Evans, R.L., D.W. Wall, C.D. Platsoucas, F.P. Siegal, S.M. Fikrig, C.M. Testa, and R.A. Good. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to antigen. Proc. Natl. Acad. Sci. USA 78:544–548 (1981).

    Article  PubMed  CAS  Google Scholar 

  57. Ledbetter, J.A., R.L. Evans, M. Lipinski, C. Cunningham-Rundles, R.A. Good, and L.A. Herzenberg. Evolutionary conservation of surface molecules that distinguish T Ijmiphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J. Exp. Med. 153:310–323 (1981).

    Article  PubMed  CAS  Google Scholar 

  58. Ledbetter, J.A., and L.A. Herzenberg. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunological Rev. 47:63–89 (1979).

    Article  CAS  Google Scholar 

  59. Nakayama, E., H. Shiku, E. Stockert, H.F. Oettgen, and L.J. Old. Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera. Proc. Natl. Acad. Sci. USA 76:1977–1981 (1979).

    Article  PubMed  CAS  Google Scholar 

  60. Shinohara, N., and D.H. Sachs. Mouse alloantibodies capable of blocking cytotoxic T-cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus. J. Exp. Med. 150:432–444 (1979).

    Article  PubMed  CAS  Google Scholar 

  61. Fan, J., A. Ahmed, and B. Bonavida. Studies on the induction and expression of T cell-mediated immunity. X. Inhibition by Lyt-2, 3 antisera of cytotoxic T lymphocyte-mediated antigen-specific and nonspecific cytotoxicity: Evidence for the blocking of the binding between T lymphocytes and target cells and not the post-binding cytolytic steps. J. Immunol. 125: 2444–2453 (1980).

    PubMed  CAS  Google Scholar 

  62. Shinohara, N., M. Taniguchi, and M. Kojima. Mouse alloantibodies capable of blocking cytotoxic T cell function. III. Studies of the mechanism of blocking of CML by anti-Lyt-2 antibodies. J. Immunol. 127:1575–1578 (1981).

    PubMed  CAS  Google Scholar 

  63. Pierres, P., C. Goridis, and P. Golstein. Inhibition of murine T cell-mediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94,000 and 180,000 molecular weight. Eur. J. Immunol., in press (1981).

    Google Scholar 

  64. MacDonald, H.R., N. Thiernesse, and J.-C. Cerottini. Inhibition of T cell-mediated cytolysis by monoclonal antibodies directed against Lyt-2: heterogeneity of inhibition at the clonal level. J. Immunol. 126:1671–1675 (1981).

    PubMed  CAS  Google Scholar 

  65. Ledbetter, J.A., W.E. Seaman, T.T. Tsu, and L.A. Herzenberg. Lyt-2 and Lyt-3 antigens are on two different polypeptide sub-units linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J. Exp. Med. 153:1503–1516 (1981).

    Article  PubMed  CAS  Google Scholar 

  66. Fan, J., and B. Bonavida. Studies on the induction and expression of T cell-mediated immunity. XII. The concomitant loss and recovery of membrane-associated Lyt-2 antigens, Ijnnphocyte-target cell binding, and the antigen-specific and-nonspecific cytotoxic activity of alloimmune T lymphocytes after treatment with trypsin. J. Immunol. 127:1856–1864 (1981).

    PubMed  CAS  Google Scholar 

  67. Dialynas, D.P., M.R. Loken, A.L. Glasebrook, and F.W. Fitch. Lyt-2−/Lyt-3− variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis. J. Exp. Mèd. 153:595–604 (1981).

    Article  PubMed  CAS  Google Scholar 

  68. Kurzinger, K., T. Reynolds, R.N. Germain, D. Davignon, E. Martz., and T.A. Springer. A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, qxiantitative expression, and structure. J. Immunol. 127:596–602 (1981).

    PubMed  CAS  Google Scholar 

  69. Davignon, D., E. Martz, T. Reynolds, K. Kurzinger, and T.A. Springer. Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1). Mechanism of blockade of T lympho-cyte-mediated killing and effects on other T and B lymphocyte functions. J. Immunol. 127:590–595 (1981).

    PubMed  CAS  Google Scholar 

  70. Shortman, K., and P. Golstein. Target cell recognition by cytolytic T cells: Different requirements for the formation of strong conjugates or for proceeding to lysis. J. Immunol. 123: 833–839 (1979).

    PubMed  CAS  Google Scholar 

  71. Martz, E. Inability of EDTA to prevent damage mediated by cytolytic T-l3rmphocytes. Cellular Immunol. 20:304–314 (1975).

    Article  CAS  Google Scholar 

  72. Springer, T.A. Mac-1, 2, 3, and 4: Murine macrophage differentiation antigens identified by monoclonal antibodies. In: “Heterogeneity of mononuclear phagocytes,” O. Forster, ed., Academic Press, New York. In press (1980).

    Google Scholar 

  73. Shinohara, N., U. Hammerling, and D.H. Sachs. Mouse allo-antibodies capable of blocking cytotoxic T cell function. II. Further study on the relationship between the blocking antibodies and the products of the Lyt-2 locus. Eur. J. Immunol. 10:589–594 (1980).

    Article  PubMed  CAS  Google Scholar 

  74. Hollander, N., E. Pillemer, and I.L. Weissman. The blocking effect of Lyt-2 antibodies on T cell functions. J. Exp. Med. 152:674–687 (1980).

    Article  PubMed  CAS  Google Scholar 

  75. Sarmiento, M., A.L. Glasebrook, and F.W. Fitch. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt-2 antigen block T cell-mediated cytolysis in the absence of complement. J. Immunol. 125:2665–2672 (1980).

    PubMed  CAS  Google Scholar 

  76. Springer, T.A., K. Kurzinger, T. Reynolds, R.N. Germain, D. Davignon, and E. Martz. MDnoclonal antibodies as probes of surface structures participating in T lymphocyte function. In: “Monoclonal Antibodies and T Cell Hybridomas,” U. Hammerling, G. Hammerling, and J. Kearney, eds., Elsevier. In press (1981).

    Google Scholar 

  77. Golstein, P., and M. Pierres. Monoclonal antibodies as probes to study the mechanism of T cell-mediated cytolysis. Proc. 14th Leukocyte Cult. Conf., in press (1981).

    Google Scholar 

  78. Lightbody, J.J., L. Urbani, and M.D. Poulik. Effect of beta-2 microglobulin antibody on effector function of T-cell mediated cytotoxicity. Nature 250:227–228 (1974).

    Article  PubMed  CAS  Google Scholar 

  79. Golstein, P. H. Wigzell, H. Blomgren, and E.A.J. Svedmyr. Cells mediating specific in vitro cytotoxicity. II. Probable autonomy of thymus-processed lymphocytes (T cells) for the killing of allogeneic target cells. J. Exp. Med. 135:890–906 (1972).

    Article  PubMed  CAS  Google Scholar 

  80. Undah K.F., and H. Lemke. Inhibition of killer-target cell interaction by monoclonal anti-H-2 antibodies. Eur. J. Immunol. 9:526–536 (1979).

    Article  Google Scholar 

  81. Epstein, S.L., K. Ozato, and D.H. Sachs. Blocking of allogeneic cell-mediated lympholysis by monoclonal antibodies to H-2 antigens. J. Immunol. 125:129–135 (1980).

    PubMed  CAS  Google Scholar 

  82. Rothstein, T.L., M.G. Mage, J. Mond, and L.L. McHugh. Guinea pig antiserum to mouse cytotoxic T lymihocytes and their precursors. J. Immunol. 120:209 (1978).

    PubMed  CAS  Google Scholar 

  83. Sullivan, K.A., G. Berke, and D.B. Amos. An antigenic determinant of cytotoxic Ijrmphocytes. Transplantation 16:388–391 (1973).

    Article  PubMed  CAS  Google Scholar 

  84. Braun, M. and F. Saal. The T-cell receptor and cytotoxicity. An anti-idiotype antiserum that inhibits a graft-versus-host reaction does not inhibit cell-mediated cytotoxicity. Cellular Immunol. 30:254–260 (1977).

    Article  CAS  Google Scholar 

  85. Lindahl, K.F. Antisera against recognition sites: lack of effect on the mixed leucocyte culture interaction. Eur. J. Immunol. 2:501–504 (1972).

    Article  PubMed  CAS  Google Scholar 

  86. Sherman, L.A., S.J. Burakoff, and B. Benacerraf. The induction of cytolytic T lymphocytes with specificity for p-azophenyl-arsonate coupled syngeneic cells. J. Immunol. 121:1432 (1978).

    PubMed  CAS  Google Scholar 

  87. Rubin, B., P. Golstein, O. Nordfang, and B. Hertel-Wulff. Generation of H-2-reactive T cell lines that bear the 5936 idiotype(s). J. Immunol. 124:161–167 (1980).

    PubMed  CAS  Google Scholar 

  88. Binz, H., and H. Wigzell. Antigen-binding, idiotypic T-lympho-cyte receptors. In: “Contemporary topics in immunobiology volume 7: T cells,” O. Stutman, Ed., Plenum Press, New York, pp. 113–177 (1977).

    Chapter  Google Scholar 

  89. Glasebrook, A.L. Conjugate formation by primary and secondary populations of murine immune T lymphocytes. J. Immunol. 121: 1870–1877 (1978).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The Department of Microbiology, University of Massachusetts, 01003, Amherst, MA, USA

    Eric Martz

  2. The Laboratory of Membrane Immunochemistry, Sidney Farber Cancer Institute, Harvard Medical School, 02115, Boston, MA, USA

    Denise Davignon, Konrad Kürzinger & Timothy A. Springer

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  1. Eric Martz
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  2. Denise Davignon
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  3. Konrad Kürzinger
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  4. Timothy A. Springer
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Editors and Affiliations

  1. University of California, Los Angeles, California, USA

    William R. Clark

  2. Centre d’lmmunologie, INSERM-CNRS de Marseille-Luminy, Marseille, France

    Pierre Golstein

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Martz, E., Davignon, D., Kürzinger, K., Springer, T.A. (1982). The Molecular Basis for Cytolytic T Lymphocyte Function: Analysis with Blocking Monoclonal Antibodies. In: Clark, W.R., Golstein, P. (eds) Mechanisms of Cell-Mediated Cytotoxicity. Advances in Experimental Medicine and Biology, vol 146. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8959-0_27

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