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Regulation of Epithelial Chloride Channels: Roles of Protein Kinases and Arachidonic Acid

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The Identification of the CF (Cystic Fibrosis) Gene

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 290))

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Abstract

Epithelial Cl- secretion plays a crucial role in controlling the content of airway fluid. The quantity and composition of airway fluid determine the effectiveness of mucociliary clearance, one of the natural lung defense mechanisms that protect the respiratory system from infection. Transcellular Cl- secretion accompanied by Na+ provides the osmotic force for water flow which hydrates the mucous coating on the surface of epithelia (see ref 1 for review).

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References

  1. Welsh, M.J., 1987, Electrolyte transport by airway epithelia, Physiol. Rev., 67:1143.

    PubMed  CAS  Google Scholar 

  2. Al-Bazzaz, F., Cheng, E., 1979, Effect of catecholamines on ion transport in dog tracheal epithelium, J. Appl. Physiol., 47:397.

    PubMed  CAS  Google Scholar 

  3. Boucher, R.C., Gatzy, J.T., 1982, Regional effects of autonomic agents on ion transport across excised canine airways, J. Appl. Physiol., 52:893.

    PubMed  CAS  Google Scholar 

  4. Davis, B., Marin, M.G., Yee, J.W., Nadel, J.A.,1979, Effect of terbutaline on movement of Cl-and Na+ across the trachea of the dog in vitro, Am. Rev. Respir. Dis., 120:547.

    PubMed  CAS  Google Scholar 

  5. Lazarus, S.C., Basbaum, C.B., Gold, W.M., 1984, Localization of cAMP in dog and cat trachea: effects of β-adrenergic agonists, Am. J. Physiol., 247:C327.

    PubMed  CAS  Google Scholar 

  6. Liedtke, C.M., Tandler, B., 1984, Physiological responsiveness of isolated rabbit tracheal epithelial cells, Am. J. Physiol., 247:C441.

    PubMed  CAS  Google Scholar 

  7. Smith, P.L. Welsh, M.J., Stoff, J.W., Frizzell, R.A., 1982, Chloride secretion by canine tracheal epithelium. I. Role of intracellular cAMP levels, J. Membr. Biol., 70:217.

    Article  PubMed  CAS  Google Scholar 

  8. Welsh, M.J., 1986, Adrenergic regulation of ion transport by primary cultures of canine tracheal epithelium: cellular electrophysiology, J. Membr. Biol., 91:121.

    Article  PubMed  CAS  Google Scholar 

  9. Al-Bazzaz, F.J., 1981, Role of cyclic AMP in regulation of chloride secretion by canine tracheal mucosa, Am. Rev. Respir. Dis., 123:295.

    PubMed  CAS  Google Scholar 

  10. Al-Bazzaz, F. J., Yadava, V.P., Westen-Felder, C., 1981, Modification of Na+ and Cl-transport in canine tracheal mucosa by prostaglandins, Am. J. Physiol., 240:F101.

    PubMed  CAS  Google Scholar 

  11. Liedtke, C.M., Boat, T.F., Rudolph, S.A., 1982, Neurohormo-7. nal receptors and cyclic AMP-binding proteins in rabbit tracheal mucosa-submucosa, Biochim. Biophys. Acta 719:169.

    Article  PubMed  CAS  Google Scholar 

  12. Barthelson, R.A., Jacoby, D.B., Widdicombe, J.H., 1987, Regulation of chloride secretion in dog tracheal epithelium by protein kinase C, Am. J. Physiol., 253:C802.

    PubMed  CAS  Google Scholar 

  13. Welsh, M.J., 1987, Effect of phorbol ester and calcium ionophore on chloride secretion in canine tracheal epithelium, Am-J. Physiol., 253:C828.

    PubMed  CAS  Google Scholar 

  14. Eling, T.E., Danilowicz, R.M., Henke, D.C., 1986, Arachidonic acid metabolism by canine tracheal epithelial cells. Product formation and relationship to chloride secretion, J. Biol. Chem., 261:12841.

    PubMed  CAS  Google Scholar 

  15. Quinton, P.M., 1983, Chloride impermeability in cystic fibrosis. Nature 301:421.

    Article  PubMed  CAS  Google Scholar 

  16. Knowles, M., Gatzy, J., Boucher, R., 1981, Increased bioelectric potential difference across respiratory epithelia in cystic fibrosis, N. Engl. J. Med., 305:1489.

    Article  PubMed  CAS  Google Scholar 

  17. Knowles, M., Gatzy, J., Boucher, R., 1983, Relative ion permeability of normal and cystic fibrosis nasal epithelium, J. Clin. Invest., 71:1410.

    Article  PubMed  CAS  Google Scholar 

  18. Knowles, M.R., Stutts, M.J., Spock, A., 1983, Abnormal ion permeation through cystic fibrosis respiratory epithelium, Science, 221:1067.

    Article  PubMed  CAS  Google Scholar 

  19. Berschneider, H.M., Knowles, M.R., Azizkhan, R.G., 1988, Altered intestinal chloride transport in cystic fibrosis, FASEB J., 2:2625.

    PubMed  CAS  Google Scholar 

  20. Widdicombe, J.H., Welsh, M.J., Finkbeiner, W.E., 1985, Cystic fibrosis decreases the apical membrane chloride permeability of monolayer cultured from cells of tracheal epithelium, Proc. Natl. Acad. Sci., 82:6167.

    Article  PubMed  CAS  Google Scholar 

  21. Yankaskas, J.R., Cotton, C.U., Knowles, M.R., 1985, Culture of human nasal epithelial cells on collagen matrix support: a comparison of bioelectric properties of normal and cystic fibrosis epithelia, Am. Rev. Respir. Dis. 132:1281.

    PubMed  CAS  Google Scholar 

  22. Yankaskas, J.R., Gatzy, J.T., Knowles, M.R., Boucher, R.C., 1985, Persistence of abnormal chloride ion permeability in cystic fibrosis nasal epithelial cells in heterologous culture, Lancet, 8435:954.

    Article  Google Scholar 

  23. Jetten, A.M., Yankaskas, J.R., Stutts, M.J., 1989, Persistence of abnormal chloride conductance regulation in transformed cystic fibrosis epithelia, Science 244.1472.

    Article  PubMed  CAS  Google Scholar 

  24. Cotton, C.U., Stutts, M.J., Knowles, M.R., 1987, Abnormal apical cell membrane in cystic fibrosis respiratory epithelium. An in vitro electrophysiological analysis, J. Clin. Invest., 79:30.

    Article  Google Scholar 

  25. Frizzell, R.A., Rechkemmer, G., Shoemaker, R.L., 1986, Altered regulation of airway epithelial cell chloride channels in cystic fibrosis, Science, 233:558.

    Article  PubMed  CAS  Google Scholar 

  26. Shoemaker, R.L., Frizzell, R.A., Dwyer, T.M., Farley, J.M., 1986, Single chloride channel currents from canine tracheal epithelial cells, Biochim. Biophys. Acta, 858:235.

    Article  PubMed  CAS  Google Scholar 

  27. Welsh, M.J., 1986, An apical-membrane chloride channel in human tracheal epithelium, Science, 232:1648.

    Article  PubMed  CAS  Google Scholar 

  28. Welsh, M. J., 1986, Single apical membrane anion channels in primary cultures of canine tracheal epithelium, Pfluegers Arch., 407:S116.

    Article  CAS  Google Scholar 

  29. Duszyk, M., French, A.S., Paul Man, S.F., 1989, The 20-pS chloride channel of the human airway epithelium, Biophys. J., 57:223.

    Article  Google Scholar 

  30. Halm, D.R., Rechkemmer, G.R., Schoumacher, R.A., Frizzell, R.A., 1988, Apical membrane chloride channels in a colonic cell line activated by secretory agonists, Am. J. Physiol., 254:CSOS.

    Google Scholar 

  31. Hayslett, J.P., Goegelein, H., Kunzelmarm, K., Greger, R., 1987: Characteristics of apical chloride channels in human colon cells (H19), Pfluegers Arch., 410:487.

    Article  CAS  Google Scholar 

  32. Krouse, M.E., Hagiwara, G., Chen, J., 1989, Ion channels in normal human and cystic fibrosis sweat gland cells, Am. J. Physiol., 257: C129.

    PubMed  CAS  Google Scholar 

  33. McCann, J.D., Li, M., Welsh, M.J., 1989, Identification and regulation of whole-cell chloride currents in airway epithelium, J. Gen. Physiol., 94:1015.

    Article  PubMed  CAS  Google Scholar 

  34. Welsh, M.J., Liedtke, C.M., 1986, Chloride and potassium channels in cystic fibrosis airway epithelia, Nature, 322:467.

    Article  PubMed  CAS  Google Scholar 

  35. Li, M., McCann, J.D., Liedtke, C.M., 1988, Cyclic AMP-dependent protein kinase opens chloride channels in normal but not cystic fibrosis airway epithelium, Nature, 3 31:358.

    Article  Google Scholar 

  36. Schoumacher, R.A., Shoemaker, R.L. Halm, D.R., 1987, Phosphorylation fails to activate chloride channels from cystic fibrosis airway cells, Nature, 330:752.

    Article  PubMed  CAS  Google Scholar 

  37. Hwang, T.C., Lu, L., Zeitlin, P., 1989, Cl-channels in CF: lack of activation by protein kinase C and cAMP-dependent protein kinase, Science, 244:1352.

    Article  Google Scholar 

  38. Walsh, P.A., Ashby, C.D., Gonzalez, C, 1985, Purification and characterization of a protein kinase inhibitor of adenosine 3’,5’-monophosphate-dependent protein kinase, J. Biol. Chem., 246:1977.

    Google Scholar 

  39. Li, M., McCann, J.D., Anderson, M.P., 1989, Regulation of chloride channels by protein kinase C in normal and cystic fibrosis airway epithelia, Science, 244:1353.

    Article  PubMed  CAS  Google Scholar 

  40. Tamaoki, T., Nomoto, H., Takahashi, I., 1986, Staurosporine, a potent inhibitor of phospholipid/Ca++ dependent protein kinase, Biochem. Biophys. Res. Commun., 135:397.

    Article  PubMed  CAS  Google Scholar 

  41. Nishizuka, Y., 1984, : The role of protein kinase C in cell surface signal transduction and tumor promotion, Nature, 308:693.

    Article  PubMed  CAS  Google Scholar 

  42. Nishizuka, Y., 1988, The molecular heterogeneity of protein kinase C and its implications for cellular regulation, Nature, 334:661.

    Article  PubMed  CAS  Google Scholar 

  43. Hwang, T.C., 1990, Ph.D. thesis: Regulation of epithelial Cl-channels: roles of protein kinases and arachidonic acid. The Johns Hopkins University, School of Medicine.

    Google Scholar 

  44. Hockberger, P., Toselli, M., Swandulla, D., Lux, H.D., 1989, A diacylglycerol analogue reduces neuronal calcium currents independently of protein kinase C activation, Nature, 338:340.

    Article  PubMed  CAS  Google Scholar 

  45. Willumsen, N.J., Boucher, R.C., 1989, Activation of an apical Cl-conductance by Ca2+ ionophores in cystic fibrosis airway epithelia, Am. J. Physiol., 256:C226.

    PubMed  CAS  Google Scholar 

  46. Widdicombe, J.H., 1986, Cystic fibrosis and β-adrenergic response of airway epithelial cell culture, Am. J. Physiol., 251:R818.

    PubMed  CAS  Google Scholar 

  47. Clancy, J.P., McCann, J.D., Li, M., Welsh, M.J., 1990, Calcium-dependent regulation of airway epithelial chloride channels, Am. J. Physiol., 258:L25.

    PubMed  CAS  Google Scholar 

  48. McPhail, L.C., Clayton, C.C., Snyderman, R., 1984, A potential second messenger role for unsaturated fatty acids: activation of Ca2+-dependent protein kinase, Science, 224:622.

    Article  PubMed  CAS  Google Scholar 

  49. Williams, J.H., Errington, M.L., Lynch, M.A., 1989, Arachidonic acid induces a longterm activity-dependent enhancement of synaptic transmission in the hippocampus, Nature, 341:739.

    Article  PubMed  CAS  Google Scholar 

  50. Barbour, B., Szatkowski, M., Ingledew, N., Attwell, D., 1989, Arachidonic acid induces a prolonged inhibition of glutamate uptake in glial cells, Nature, 342:918.

    Article  PubMed  CAS  Google Scholar 

  51. Kim, D., Clapham, D.E., 1989, Potassium channels in cardiac cells activated by arachidonic acid and phospholipids, Science, 244:1174.

    Article  PubMed  CAS  Google Scholar 

  52. Ordway, R.W., Walsh, J.V., Singer, J.J. Jr., 1989, Arachidonic acid and other fatty acids directly activate potassium channels in smooth muscle cells, Science, 244:1176.

    Article  PubMed  CAS  Google Scholar 

  53. Sarkadi, B., Cheung, R., Mack, E., 1985, Cation and anion transport pathways in volume regulatory response of human lymphocytes to hyposmotic media, Am. J. Physiol., 248:C480.

    PubMed  CAS  Google Scholar 

  54. Landry, D.W., Reitman, M., Cragoe, E.J. Jr., Al-Awqati, Q., 1987, Epithelial chloride channel: Development of inhibitory ligands, J. Gen. Physiol., 90:779.

    Article  PubMed  CAS  Google Scholar 

  55. Hwang, T.C., Guggino, S.E., Guggino, W.B., Direct modulation of secretory Cl-channels by arachidonic acid, Accepted for publication by Proc. Natl. Acad. Sci. USA.

    Google Scholar 

  56. Dharmsathaphorn, K., McRoberts, J.A., Mandei, K.G., 1984, A human colonic tumor cell line that maintains vectorial electrolyte transport, Am. J. Physiol., 246:G204.

    PubMed  CAS  Google Scholar 

  57. Dahlen, S.-E., Hansson, G., Hedqvist, P., 1983, Allergen challenge of lung tissue from asthmatics elicits bronchial contraction that correlates with the release of leukotriene C4, D4 and E4, Proc. Natl. Acad. Sci. USA, 80:1712.

    Article  PubMed  CAS  Google Scholar 

  58. Weiss, J.W., Drazen, J.M., Coles, N., 1982, Bronchoconstrictor effects of leukotriene C in humans, Science, 216:196.

    Article  PubMed  CAS  Google Scholar 

  59. Hardy, C.C., Robinson, C., Tattersfield, A.E., Holgate, S.T., 1984, The bronchosonstrictor effect of inhales prostaglandin D2 in normal and asthmatic men, N. Engl. J. Med., 311:209.

    Article  PubMed  CAS  Google Scholar 

  60. Carlstedt-Duke, J., Broennegard, M., Strandvik, B., 1986, Pathological regulation of arachidonic acid release in cystic fibrosis: the putative basic defect, Proc. Natl. Acad. Sci. USA, 83:9202.

    Article  PubMed  CAS  Google Scholar 

  61. Lewis, R.A., Soter, N.A., Diamond, P.T., 1982, Prostaglandin D2 generation after activation of rat and human mast cells with anti-IgE, J. Immun., 129:1627.

    PubMed  CAS  Google Scholar 

  62. Churchill, L., Chilton, F.H., Resau, J.H., 1989, Cyclooxygenase metabolism of endogenous arachidonic acid by cultured human tracheal epithelial cells, Am. Rev. Respir. dis., 140:449.

    Article  PubMed  CAS  Google Scholar 

  63. MacDermot, J., Barnes, P.J., 1980, Activation of guinea pig pulmonary adenylate cyclase by prostacyclin, Eur. J., Pharmacol., 67:419.

    Article  CAS  Google Scholar 

  64. Hille, B., 1984, Ionic Channels in Excitable Membranes, Sinauer Association Inc., Massachusetts.

    Google Scholar 

  65. White, M.M., Miller, C., 1981, Probes of the conduction process of a voltage-gated Cl-channel from Torpedo electro-plax, J. Gen. Physiol., 78:1.

    Article  PubMed  CAS  Google Scholar 

  66. Bahinski, A., Nairn, A.C., Greengard, P., Gadsby, D.C., 1989, Chloride conductance regulated by cyclic AMP-dependent protein kinase in cardiac myocytes, Nature, 340:718.

    Article  PubMed  CAS  Google Scholar 

  67. Levitan I.B., 1988, Modulation of ion channels in neurons and other cells, Ann. Rev. Neurosci., 11:119

    Article  PubMed  CAS  Google Scholar 

  68. Bean, B.P., Nowycky, M.C., Tsien, R.W., 1984, β-adrenergic modulation of calcium channels in frog ventricular heart cells, Nature 307:371.

    Article  PubMed  CAS  Google Scholar 

  69. Cachelin, A.B., de Peyer, J.E., Kokubun, S., Reuter, H., 1983, Ca2+ channel modulation by 8-bromocyclic AMP in cultured heart cells, Nature, 304:462.

    Article  PubMed  CAS  Google Scholar 

  70. Soliven, B., Szuchet, S., Arnason, B.G.W., Nelson, D.J., 1988, Forskolin and phorbol esters decrease the same K+ conductance in cultured oligodendrocytes, J. Membr. Biol., 105:177.

    Article  PubMed  CAS  Google Scholar 

  71. Sims, S.M., Singer, J.J., Walsh, J.V. Jr., 1988, Antagonistic adrenergic-muscarinic regulation of M current in smooth muscle cells, Science, 239:190.

    Article  PubMed  CAS  Google Scholar 

  72. Kume, H., Takai, A., Tokuno, H., Tomita, T., 1989, Regulation of Ca2+-dependent K+ channel activity in tracheal myocytes by phosphorylation, Nature, 341:152.

    Article  PubMed  CAS  Google Scholar 

  73. Nastainczyk, W., Roehrkasten, A., Sieber, M., 1987, Phosphorylation of the purified receptor for calcium channel blockers by cAMP kinase and protein kinase C, Euro. J. Biochem., 169:137.

    Article  CAS  Google Scholar 

  74. Huganir, R.L., Greengard, P., cAMP-dependent protein kinase phosphorylates the nicotinic acetylcholine receptor, Proc. Natl. Acad. Sci. USA, 80:1130.

    Google Scholar 

  75. Miles, K., Anthony, D.T., Ruben, L.L., 1987, Regulation of nicotinic acetylcholine receptor phosphorylation in rat myotubes by forskolin and cAMP, Proc. Natl. Acad. Sci. USA, 84:6591.

    Article  PubMed  CAS  Google Scholar 

  76. Walsh, K.B., Kass, R.S., 1988, Regulation of a heart potassium channel by protein kinase A and C, Science, 242:67.

    Article  PubMed  CAS  Google Scholar 

  77. Hammond, C., Paupardin-Tritsch, D., Nairn, A.C., 1987, Cholecystokinin induces a decrease in Ca2+ current in snail neurons that appears to be mediated by protein kinase C, Nature, 325:809.

    Article  PubMed  CAS  Google Scholar 

  78. Lacerda, A.E., Rampe, D., Brown, A.M., 1988, Effects of protein kinase C activators on cardiac Ca2+ channels, Nature, 335:249.

    Article  PubMed  CAS  Google Scholar 

  79. Strong, J.A., Fox, A.P., Tsien, R.W., Kaczmarek, L.K., 1987 Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons, Nature, 325:714.

    Article  PubMed  CAS  Google Scholar 

  80. Miles, K., Huganir, R.L., 1988, Regulation of nicotinic acetylcholine receptors by protein phosphorylation, Mol. Neuro-biol. 2:91.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, The Johns Hopkins University, USA

    Tzyh-Chang Hwang & William B. Guggino

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  1. Tzyh-Chang Hwang
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  2. William B. Guggino
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Editors and Affiliations

  1. The Hospital for Sick Children, Toronto, Ontario, Canada

    Lap-Chee Tsui

  2. Istituto Giannina Gaslini, Genoa, Italy

    Giovanni Romeo

  3. Albert-Ludwigs-Universität, Freiburg, Germany

    Rainer Greger

  4. Fondazione Internazionale Menarini, Florence, Italy

    Sergio Gorini

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Hwang, TC., Guggino, W.B. (1991). Regulation of Epithelial Chloride Channels: Roles of Protein Kinases and Arachidonic Acid. In: Tsui, LC., Romeo, G., Greger, R., Gorini, S. (eds) The Identification of the CF (Cystic Fibrosis) Gene. Advances in Experimental Medicine and Biology, vol 290. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5934-0_21

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  • DOI: https://doi.org/10.1007/978-1-4684-5934-0_21

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