Summary
The effect of various extracellular phosphate concentrations on glucose induced insulin secretion has been investigated in two experimental systems the isolated perfused rat pancreas and the isolated rat pancreatic islets incubated in vitro. In both systems, total insulin secretion in response to 16.7 mmol/1 glucose increased as extracellular phosphate concentration increased from 0 to 3.6 mmol/. In perfusion experiments insulin secretion was significantly reduced at 4.8 mmol/1 phosphate. In incubation experiments insulin secretion was inhibited only at 9.6 mmol/1 phosphate concentration. When IBMX 1 mmol/1 was used in incubation experiments glucose induced insulin secretion increased as extracellular phosphate increased from 0 to 3.6 mmol/1, but was also inhibited at 4.8 mmol/1.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
- Borle; A.S. Calcium metabolism at the cellular level. Fed. Proc. 32, 1944–1950, 1973.
- Borowitz, J.L. and Matthews, E.K. Calcium exchangeability in subcellular fractions of pancreatic islet cells. J. Cell. Sci., 41, 233–243, 1980.
- Bukowiecki, L. and Freinkel, N. Relationship between efflux of ionic calcium and phosphorus during excitation of pancreatic islets with glucose. Biochim. Biophys. Res. Commun. 63, 870–874, 1976.
- Campillo, J.E., Luyckx, A.S., Torres, M.D. and Lefebvre, P. J. Effect of phosphate omission on arginine-induced insulin and glucagon release by the isolated perfused rat pancreas. FEBS. Letts. 34, 141–143, 1977.
- Campillo, J.E., Luyckx, A.S., Torres, M.D. hnd Lefebvre, P. J. Effect of various concentrations of calcium on arginine induced insulin and glucagon release in vitro. Rev. Esp. Physiol., 34, 191–198, 1978.
- Campillo, J.E., Castillo, M., Valdivia, M. and Rodriguez, E. Effect of phosphate omission on glucose induced insulin release in vitro. Experientia, 35, 1260–1261, 1979.
- Campillo, J.E., Osuna, J.I. Pages, M.T., Blazquez; A., CASTILLO, M. and OSORIO, C. Effect of inorganic phosphate on basal and glucose induced insulin release in vitro. Influence of magnesium concentration. Diabetologia, 21, 256–257 (Abstract) (1981).
- Dean, P.M. Ultrastructural morphometry of the pancreatic B-cells. Diabetologia, 9, 115–119, 1973.
- Sehlin, J. Univalent ions in islet function. Horm. Metab. Res. 10 (Suppl), 73–80, 1980.
-Frfinkel, N., Pedley, K.C., Wooding, P. and Dawson, R.M.C. Localization of inorganic phosphate in the pancreatic B-cell and its less on glucose stimulation. Science, 201, 1124–1126, 1978.
-Hales, C.N. and Randle; P.J. Immunoassay of insulin with insulin antibody precipitate. Biochem. J., 88, 137–146, 1963.
-Hellman, B. and Andersson, T. Calcium and pancreatic B-cell function. 4. Evidence that glucose and phosphate stimulate calcium-45 incorporation in to different intracellular pools. Biochim. Biophys. Acta, 241, 423–491, 1978.
- Kikuchi, M., Wollheim, C.B., Siegel, E.G., Renold, A. E. and Sharp, G.W.G. Biphasic insulin release in rat islets of Langerhans and the role of intracellular calcium stores. Endocrinology, 105, 1013 1019, 1979.
- Lacy, P. E. and Kostianowsky, M. Methods for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes, 16, 35–39, 1967.
- Malaisse, W.J., Hutton, J.C., Kaiwazu, S., Herchuelz, A., Valverde, I. and Sener, A. The stimulus secretion coupling of glucose-induced insulin release. XXV. The links between metabolic and cationic events. Diabetologia, 16, 325–330, 1979.
- Marshall, W.P., Banasiak, M.F. and Kalkhoff, P.K. Effects of phosphate deprivation on carbohydrate metabolism. Horm. Met. Res. 10, 369–375, 1978.
- Rasmussen, H., Goodman, D.R.P. Relationship between calcium and cyclic nucleotides in cell activation. Physiol. Rev. 57, 421–509, 1977.
- Ravazzola; M., M.laisse-Lagae, F., Amherotm, M., Malaisse; W.J. and Orci, L. Patterns of calcium localization in pancreatic endocrine cells. J. Cell Sci., 27, 107–117, 1976.
- Freinkel, N., EL Younsi, C., Bonnar, J. and Dawson, M.C. Rapid transient efflux of phosphate ions from pancreatic islets as an early action of insulin secretagogues. J. Clin. Invest. 54, 1179 1180, 1974.
- Sugden, M.C. and Ashcroft, S.J.H. Effects of phosphoenolpyruvate, other glycolitic intermediates and methylxantines on calcium uptake by a mitochondrial fraction from rat pancreatic islets. Diabetologia, 15, 173–180, 1978.
-Wollheim, C.B. and Sharp, G.W.G. Regulation of insulin release by calcium. Physiol. Rev. 61, 914–973, 1981.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Osuna, J.I., Castillo, M., Rodriguez, E., Campillo, J.E., Osorio, C. (1986). Influence of Inorganic Phosphate on Glucose-Induced Insulin Release in Vitro. In: Massry, S.G., Olmer, M., Ritz, E. (eds) Phosphate and Mineral Homeostasis. Advances in Experimental Medicine and Biology, vol 208. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5206-8_63
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5206-8_63
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5208-2
Online ISBN: 978-1-4684-5206-8
eBook Packages: Springer Book Archive