Abstract
The kidney cortex is a highly aerobic and metabolically active tissue rich in mitochondria and Na-and K-dependent adenosine triphosphatase (Na,K-ATPase) activity. The basal respiration rate of a suspension of rabbit proximal tubules utilizes 50-60 percent of the mitochondrial respiratory capacity (Harris et al., 1981). Since Na,K-ATPase-mediated ion transport consumes more metabolic energy than any other single enzymatic process within the mammalian organism, it is not surprising that rabbit proximal tubules utilize half of their basal respiration for this process (Cohen and Kamm, 1976; Harris et al., 1981). The remaining half of their basal respiration is divided between ATP generation for other processes and nonphosphorylating respiration. Using isolated mitochondria, nonphosphorylating respiration has been shown to be 5–8 percent of the basal respiration (Davis et al., 1974).
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References
Bergmeyer, H.-U., Bernt, E., and Hess, B., 1973, Lactic dehydrogenase, in: “Methods of Enzymatic Analysis,” H.-U. Bergmeyer, ed., Academic Press, London.
Chance, B. and Williams, G.R., 1956, The respiratory chain and oxidative phosphorylation. Adv. Enzymol., 17: 65.
Cohen, J.J. and Kamm, D.E., 1976, Renal metabolism: relation to renal function, in: “The Kidney,” B.M. Brenner and F.C. Rector, eds., Saunders, Philadelphia.
Davis, J.E., Lumeng, L., and Bottoms, D., 1974, On the relationships between the stoichiometry of oxidative phosphorylation and the phosphorylation potential of rat liver mitochondria as functions of respiratory rate, FEBS Lett., 39: 9.
Harris, S.I., Balaban, R.S., Barrett, L., and Mandel, L.J., 1981, Mito-chondrial respiratory capacity and Na+- and K+-dependent adenonsine triphosphatase-mediated ion transport in the intact renal cell, J. Biol. Chem., 256: 10319.
Gornall, A.G., Bardawill, C.J., and David, M.M., 1949, Determination of serum proteins by means of a biuret reaction, J. Biol. Chem., 177: 751.
Lau, S.S., Monks, T.J., and Gillette, J.R., 1984, Identification of 2bromohydroquinone as a metabolite of bromobenzene and o-bromophenol: Implications for bromobenzene-induced nephrotoxicity, J. Pharmacol. Exp. Ther., 230: 360.
Schnellmann, R.G. and Mandel, L.J., 1985a, Multiple effects of presumed glutathione depletors on rabbit proximal tubules, Kidney Int. (in press)
Schnellmann, R.G. and Mandel, L.J., 1985b, Cellular toxicity of bromobenzene and bromobenzene metabolites to rabbit proximal tubules: The role and mechanism of 2-bromohydroquinone, J. Pharmacol. Exp. Ther. (submitted).
Soltoff, S.P. and Mandel, L.J., 1984, Active ion transport in the renal proximal tubule, J. Gen. Physiol., 84: 601.
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© 1986 Plenum Press, New York
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Schnellmann, R.G., Mandel, L.J. (1986). Inhibition of Respiration in Rabbit Proximal Tubules by Bromophenols and 2-Bromohydroquinone. In: Kocsis, J.J., Jollow, D.J., Witmer, C.M., Nelson, J.O., Snyder, R. (eds) Biological Reactive Intermediates III. Advances in Experimental Medicine and Biology, vol 197. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5134-4_87
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DOI: https://doi.org/10.1007/978-1-4684-5134-4_87
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