Abstract
Since the initial observations (1,2) of the effect of 2,3 diphosphoglycerate (2,3 DPG) upon the oxygen affinity of hemoglobin, considerable interest has focussed on the importance in the gas exchange process (3,4) of this species and other organic phosphates. This paper summarizes a theoretical analysis of the influence of 2,3 DPG on O2 transport in hemoglobin solutions. The physical system analyzed is the same as that used in previous treatments of hemoglobin-facilitated oxygen transfer (5). A thin film of solution separates two phases containing differing partial pressures of oxygen (PO2). Hemoglobin, although mobile, is constrained to remain within the liquid film. As a result of 02-Hb interactions, the diffusion of O2 from the high to the low pO2 boundary induces a parallel diffusion of oxyhemoglobin. If 2,3 DPG is present, there are gradients in the concentrations of free and of hemoglobin-bound 2,3 DPG as a result of the gradients in oxy- and deoxyhemoglobin and preferential binding of 2,3 DPG to the latter (6). In this study, the concentrations of free and bound 2,3 DPG were taken to be constant across the film.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Benesch, R. and Benesch, R.E. (1967) Biochem. Biophys. Res. Commun. 26, 162.
Chanutin, A. and Curnish, R.R. (1967) Arch. Biochem. Biophys. 121, 96.
Forster, R.E. (1972) in Benzon Symp. IV, Rorth, M. and Astrup, P., eds., Munskgaard, Copenhagen, p. 518.
Rossi-Bernardi, L., et al. (1972) in Benzon Symp. IV, Rorth, M. and Astrup, P., eds., Munskgaard, Copenhagen, p. 224.
Kreuzer, F. (1970) Resp. Physiol. 9, 1.
Perutz, M.F. (1970) Nature, 226, 726.
Olander, D.R. (1960) AIChE J. 6, 233.
Kreuzer, F. and Hoofd, L.J.C. (1972) Resp. Physiol. 15, 104.
Kutchai, J., Jacquez, J.A., and Mather, F.J. (1970) Biophys. J. 10, 38.
Adair, G.S. (1925) J. Biol. Chem. 63, 529.
Tyuma, I., Imai, K., and Schimizu, K. (1972) in Benzon Symp. IV, Rorth, M. and Astrup, P., eds., Munskgaard, Copenhagen, p. 139.
Gibson, Q.H. (1970) J. Biol. Chem. 245, 3285.
Meldon, J.H. (1973) Sc.D. Thesis, MIT, Cambridge.
Smith, K.A., Meldon, J.H., and Colton, C.K. (1973) AIChE J. 19, 102.
Gibson, Q.H. (1973) Proc. Nat. Acad. Sci. 70, 1.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1973 Springer Science+Business Media New York
About this chapter
Cite this chapter
Meldon, J.H., Smith, K.A., Colton, C.K. (1973). Analysis of 2,3 Diphosphoglycerate-Mediated, Hemoglobin-Facilitated Oxygen Transport in Terms of the Adair Reaction Mechanism. In: Bicher, H.I., Bruley, D.F. (eds) Oxygen Transport to Tissue. Advances in Experimental Medicine and Biology, vol 37 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3288-6_25
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3288-6_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3290-9
Online ISBN: 978-1-4684-3288-6
eBook Packages: Springer Book Archive