Abstract
An endonuclease that makes single polynucleotide chain scissions in UV-irradiated DNA has been purified from Escherichia coli. The activity has the following properties: (1) unirradiated DNA is attacked very little if at all; (2) single-stranded DNA is not attacked, whether irradiated or not; (3) there is no requirement for divalent cations, and the activity is not affected by the addition of EDTA; (4) the pH optimum is approximately 7; (5) the activity is inhibited by 1 M NaC1, single-stranded DNA, transfer RNA, and unirradiated double-stranded DNA; (6) the sedimentation coefficient, S 20, W’ is approximately 2.6; (7) it is a basic protein. The enzyme is tentatively named E. coli endonuclease III. The physiological function of the endonuclease has not yet been established.
A full report will be published elsewhere (Radman, 1975).
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© 1975 Plenum Press, New York
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Radman, M. (1975). Endonuclease III: An Endonuclease from Escherichia coli That Introduces Single Polynucleotide Chain Scissions in Ultraviolet-Irradiated DNA. In: Hanawalt, P.C., Setlow, R.B. (eds) Molecular Mechanisms for Repair of DNA. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2895-7_24
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DOI: https://doi.org/10.1007/978-1-4684-2895-7_24
Publisher Name: Springer, Boston, MA
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