Abstract
A serious drawback in neurochemistry still is the difficulty to resolve the complexity of cell types involved in the neuronal and glial network of brain into pure cell fractions. Although a number of procedures for the separation of neuronal perikaria and glial cell types have been described, the axons and dendrites of these cells are sheared off and this may be accompanied by a leakage of cell components during isolation with all consequences (l–4). A recent new concept in neurochemistry has arisen from the availability of clonal cell lines of different CNS and PNS stams (5–9), notably neuroblastoma by Augusti-Tocco and Sato, and chemically induced glioma and Schwann cell tumors by Pfeiffer and Wechsler. These cells grow as permanent cell lines and can be easily characterized eIectrophysioIogicaI Iy and biochemically; moreover their properties remain stable over the generations sofar observed. The availability of these cell cultures for biochemical studies induced us to study their phospholipid and glycolipid metabolism
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Binaglia, L., Karageosian, K., Stoffel, W. (1976). Phospho- and Glycosphingolipid Metabolism in a Rat Neurinoma Clone (RN6) Studied in Tissue Culture. In: Porcellati, G., Amaducci, L., Galli, C. (eds) Function and Metabolism of Phospholipids in the Central and Peripheral Nervous Systems. Advances in Experimental Medicine and Biology, vol 72. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0955-0_12
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DOI: https://doi.org/10.1007/978-1-4684-0955-0_12
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